概述

  • 产品名称Anti-E. coli抗体
    参阅全部 E. coli 一抗
  • 描述
    山羊多克隆抗体to E. coli
  • 特异性ab25823 recognises all E. coli species tested. The following species of E. coli were used in the antigen prep: Heat killed sonicate of E. coli from K12, TOP 10F, XS127/P3, JM109,HB101, and DH5a and BL21. We have also extensively tested K-12 Since there is ]95% homology between the strains, all species of E.coli should react with this antibody.
  • 经测试应用适用于: ICC/IF, Electron Microscopy, WB, ELISA, Dot blotmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Tissue/ cell preparation (Escherichia coli ): Heat killed sonicate of E. coli (cocktail blend of strains of E. coli including the most commonly used DH5a and BL21).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 150mM Sodium chloride, ~25mM HEPES, pH 7.0 to 7.4
    The buffer contains trace amounts of citric acid and sodium phosphate. There are no preservatives or any other amine containing compounds and as such this material can be directly conjugated to enzymes, biotin etc. by most chemistries without the
    need to diafilter.
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22427638
Electron Microscopy Use at an assay dependent concentration. PubMed: 24253282
WB Use a concentration of 0.5 - 2 µg/ml. Note: Reacts with more than 50 proteins by Western Blot.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

靶标

  • 相关性Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • 别名
    • Escherichia coli antibody

Anti-E. coli antibody (ab25823)参考文献

This product has been referenced in:
  • Nayak M  et al. Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms. Sci Rep 3:3266 (2013). Electron Microscopy ; Escherichia coli . Read more (PubMed: 24253282) »
  • So NS  et al. Vigorous response of human innate functioning IgM memory B cells upon infection by Neisseria gonorrhoeae. J Immunol 188:4008-22 (2012). ICC/IF . Read more (PubMed: 22427638) »

See all 5 Publications for this product

Product Wall

Abreviews
Application ELISA
Sample Escherichia coli Cell (K12 bacterial solution)
Specification K12 bacterial solution
Type Other
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 May 13 2014


The Goat polyclonal anti-E. coli conjugated to HRP is ab69185. The link to the datasheet is http://www.abcam.com/ab69185.

Thank you for contacting us.

We only supply this antibody in 100 ug sizes however, if you were to purchase 5 or more vials of this product, it would be possible for me to give you a discount.

If you could let me know how many vial...

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Thank you for contacting us.


The K12 strain was one of the strains used to generate the antibody.

This antibody was actually raised against a blend of lysates from both BL21 and DH5a and as a result will detect the majority of...

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Thank you for contacting Abcam.

The SDS for ab25823 has been created and will be posted on the website by the "Datasheet PDF" tab within the next 48 hours (http://www.abcam.com/e-coli-antibody-ab25823.html).

I hope this info...

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Thank you for your email.

I understand your concerns that antibodies should form immune complex with compatible antigens irrespective of their location however unfortunately this is not the case. Antibodies behaves differently in each applicat...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting us.

I have heard back from the lab with the following answers:

1. ab25823 can be used for Western blot in the concentration range of 0.5 to 2ug/mL.

2. We have not tested the O157 strain for cross...

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Merci de nous avoir contactés.
Comme expliqué par téléphone j'ai mis en pièce jointe un questionnaire qui nous permettra de regarder plus en détails votre protocole.
Les liens vers les protocoles Abcam pour la cytométrie en flux sont:
> htt...

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1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"