Anti-E Cadherin抗体[HECD-1] (ab1416)

概述

  • 产品名称
    Anti-E Cadherin抗体[HECD-1]
    参阅全部 E Cadherin 一抗
  • 描述
    小鼠单克隆抗体[HECD-1] to E Cadherin
  • 经测试应用
    适用于: Flow Cyt, ICC, IP, IHC-P, IHC-Fr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    不与反应: Mouse
  • 免疫原

    Breast tumor cell line MCF-7

  • 阳性对照
    • Breast Cancer

性能

应用

Our Abpromise guarantee covers the use of ab1416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 19244118
WB 1/50.

靶标

  • 功能
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • 组织特异性
    Non-neural epithelial tissues.
  • 疾病相关
    Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • 序列相似性
    Contains 5 cadherin domains.
  • 翻译后修饰
    During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • 细胞定位
    Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody [HECD-1] 图像

  • ab1416 staining E cadherin in Human renal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.01% BSA) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • Breast cancer stained with E Cadherin mouse antibody ab1416

  • Lanes 1 - 2 : Anti-E Cadherin antibody [HECD-1] (ab1416) at 1/50 dilution
    Lanes 3 - 4 : Anti-GAPDH at 1 µg/ml

    Lane 1 : MDA-MB-231 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : MDA-MB-231 cell lysate
    Lane 4 : MCF-7 cell lysate

    Blocking buffer: 3% milk

  • Overlay histogram showing MCF7 cells stained with ab1416 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1416, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab1416 staining E Cadherin in Human nasal epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed paraformaldehyde and permeabilized with Triton X-100 0.1%. Samples were incubated with primary antibody (1/50) for 18 hours at 4°C. A Cy2®-conjugated Horse anti-mouse monoclonal(1/100) was used as the secondary antibody.

    See Abreview

  • ab1416 staining E Cadherin in human normal skin tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone. Samples were blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/50 dilution for 30 minutes at 21°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    See Abreview

  • All lanes : Anti-E Cadherin antibody [HECD-1] (ab1416) at 1/1000 dilution

    Lane 1 : MDA-MB-468 cells whole cell lysate
    Lane 2 : MDA-MB-468 cells whole cell lysate

    Lysates/proteins at 80 µg/ml per lane.

    Secondary
    HRP-conjugated polyclonal donkey anti-mouse IgG

    Performed under reducing conditions.

    Observed band size : 110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Dr Alban Gaultier

    See Abreview

  • ab1416 staining E Cadherin in the Human NSCLC cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton in PBS and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 0.3% Triton, 5% Goat Serum in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • ab1416 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

    See Abreview

  • ab1416 staining E Cadherin in Human Renal Proximal Tubular cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton ×100 and blocking with 1% BSA was done for 30 minutes at 210C. Samples were incubated with primary antibody (1/50: in 0.1 % BSA in PBS) for 24 hours at 4°C. An Alexa Fluor®488-conjugated goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

    See Abreview

Anti-E Cadherin antibody [HECD-1] (ab1416)参考文献

This product has been referenced in:
  • Lin K  et al. Silencing of SRA1 Regulates ER Expression and Attenuates the Growth of Stromal Cells in Ovarian Endometriosis. Reprod Sci 24:836-843 (2017). Read more (PubMed: 27694140) »
  • Sioutas A  et al. Oxidant-induced autophagy and ferritin degradation contribute to epithelial-mesenchymal transition through lysosomal iron. J Inflamm Res 10:29-39 (2017). WB ; Human . Read more (PubMed: 28405169) »

See all 105 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Cow Tissue sections (Fetal Heart)
Specification
Fetal Heart
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Juliana Shimara Pires Ferrão

Verified customer

提交于 Nov 26 2014

Thank you for sending the image. It may well be that the vial you have received was a bad vial which is why the antibody is not detecting the target although it is very strictly qc tested. If you are interested in trying the same antibody I can sen...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 26 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 26 2017

Application
Western blot
Sample
Human Cell lysate - other (LNCaP - prostate cancer cells - plasma membrane fr)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
25 µg
Specification
LNCaP - prostate cancer cells - plasma membrane fr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 26 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Human Cell (Renal tubular epithelial cells)
Specification
Renal tubular epithelial cells
Permeabilization
Yes - Triton X100, 0.5 %
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human NSCLC Cell Line)
Specification
Human NSCLC Cell Line
Permeabilization
Yes - 0.3% Triton in PBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
Methanol
Permeabilization
No
Sample
Human Cell (A431 cells)
Specification
A431 cells
Gating Strategy
no gate
Preparation
Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
Sample buffer: PBS/10% human IgG
Username

Abcam user community

Verified customer

提交于 Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (nasal epithelial cells)
Specification
nasal epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100 0.1%
Username

Mr. Michael Könnecke

Verified customer

提交于 Feb 13 2013

Muchas gracias por enviarnos el cuestionario relleno con la información del anticuerpo.

Siento que el anticuerpo no haya dado los resultados esperados en ninguna de las técnicas utilizadas, y espero poder ayudar a su optimizaci&...

Read More

1-10 of 57 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册