RabMAb

Anti-E Cadherin抗体[EP700Y] - Intercellular Junction Marker (ab40772)

概述

  • 产品名称Anti-E Cadherin抗体[EP700Y] - Intercellular Junction Marker
    参阅全部 E Cadherin 一抗
  • 描述
    兔单克隆抗体[EP700Y] to E Cadherin - Intercellular Junction Marker
  • 特异性This antibody has been tested on human samples in both WB and IHC. However,mouse samples have only been tested in WB in the laboratory; customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.
  • 经测试应用适用于: IHC-Fr, ICC/IF, IHC-P, IP, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the 5th domain of human E-cadherin

  • 阳性对照
    • MCF-7 cell lysate; human breast carcinoma; human hepatocellular carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-E Cadherin antibody (Alexa Fluor® 488) [EP700Y] - Intercellular Junction Marker (ab185013)

    Anti-E Cadherin antibody (Alexa Fluor® 555) [EP700Y] - Intercellular Junction Marker (ab206878)

    Anti-E Cadherin antibody (Alexa Fluor® 568) [EP700Y] - Intercellular Junction Marker (ab206879)

    Anti-E Cadherin antibody (Alexa Fluor® 594) [EP700Y] - Intercellular Junction Marker (ab206880)

    Anti-E Cadherin antibody (Alexa Fluor® 647) [EP700Y] - Intercellular Junction Marker (ab194982)

性能

应用

Our Abpromise guarantee covers the use of ab40772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24915897
ICC/IF 1/500.

Permeabilisation is unnecessary as the immunogen is in an extracellular domain.

IHC-P 1/500.
IP 1/50.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/10000 - 1/50000. Predicted molecular weight: 120 kDa.

靶标

  • 功能Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
  • 组织特异性Non-neural epithelial tissues.
  • 疾病相关Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
    Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
  • 序列相似性Contains 5 cadherin domains.
  • 翻译后修饰During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions.
  • 细胞定位Cell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arc 1 antibody
    • CADH1_HUMAN antibody
    • Cadherin 1 antibody
    • cadherin 1 type 1 E-cadherin antibody
    • Cadherin1 antibody
    • CAM 120/80 antibody
    • CD 324 antibody
    • CD324 antibody
    • CD324 antigen antibody
    • cdh1 antibody
    • CDHE antibody
    • E-Cad/CTF3 antibody
    • E-cadherin antibody
    • ECAD antibody
    • Epithelial cadherin antibody
    • epithelial calcium dependant adhesion protein antibody
    • LCAM antibody
    • Liver cell adhesion molecule antibody
    • UVO antibody
    • Uvomorulin antibody
    see all

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker 图像

  • Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab40772. Green-E-Cadherin red-PI

  • ab40772 showing positive staining in human colonic adenocarcinoma tissue.

  • Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) at 1/200000 dilution + MCF7 (Human breast adenocarcinoma ) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

    Predicted band size : 120 kDa
    Observed band size : 80,97,100,120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Blocking and diluting buffer and concentration 5% NFDM/TBST.

    The full-length of E-cadherin is 120 kDa. The other bands are due to proteolytic cleavages in different Cadherin domains. (Ref: PMID: 14695147)

  • ICC/IF image of ab40772 stained human MCF7 cells. The cells were fixed in Paraformaldehyde, before incubation in 0.1% BSA + 1% goat serum in PBS as a blocking agent for 30 mins at 22oC. The cells were then incubated with the antibody (ab40772, 1:100 dilution) for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • Overlay histogram showing A431 cells stained with ab40772 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40772, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab40772 showing positive staining in human lung adenocarcinoma tissue.

  • ab40772 showing positive staining in human breast carcinoma tissue.

  • ab40772 showing positive staining in human papillary carcinoma of thyroid gland tissue.

  • ab40772 showing positive staining in human transitional cell carcinoma of kidney tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772)参考文献

This product has been referenced in:
  • Su B  et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016). Read more (PubMed: 26871290) »
  • Schmidt TP  et al. Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA. Sci Rep 6:23264 (2016). WB ; Human . Read more (PubMed: 26983597) »

See all 40 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

提交于 Jul 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Pluripotent stem cell)
Permeabilization Yes - 0.1% Triton X100
Specification Pluripotent stem cell
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Oliver Thompson

Verified customer

提交于 Apr 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6
Sample Human Tissue sections (colon)
Specification colon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 10 2015



This antibody recognizes an extracellular epitope on E-cadherin.

Thank you for contacting us.

We do not provide discount for already tested applications or species. The product is guaranteed working in ICC/IF and will work irrespective of the cell line customer is using, required the cell line express the p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF-7)
Specification MCF-7
Fixative Paraformaldehyde
Permeabilization No
Blocking step 0.1% BSA + 1% goat serum in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF-7)
Loading amount 100000 cells
Specification MCF-7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (vaginal epithelial cells)
Loading amount 40 µg
Specification vaginal epithelial cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 06 2012

Thank you for your enquiry and your interest.
We provide the immunogen details on the product datasheets unless the sequence information is confidential.
I regret to inform you that for these three antibodies, we are unable to release the exa...

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Thank you for contacting us.

I do not see a reason why a chromogenic detection method in ICC should not work. The protocol will certainly requireoptimisation though, but you may use a chromogenicdetection method to visualise the expression of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"