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Our Abpromise guarantee covers the use of ab15148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 120 kDa.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
ab15148 staining E Cadherin in Human breast cancer MDA-MB-231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with ehanol (vehicle) as control or Origanummarjorana extract for 24 hours. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature. Samples were incubated with primary antibody overnight at 4°C. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% BSA fro 1 hour at 23°C
ab15148 staining human MCF10A cells by ICC/IF. Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C. The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.
ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C. An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.
ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
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