概述

  • 产品名称
  • 描述
    山羊多克隆抗体to DYX1C1
  • 特异性
    This antibody is expected to recognise only one of the three reported isoforms of DYX1C1, isoform a.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Human, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-KIRNVIQGTELKS

    , corresponding to amino acids 408-420 of DYX1C1

  • 阳性对照
    • Human brain lysate. Lysates of COS1 cells transfected with full length recombinant Human DYX1C1.

性能

应用

Our Abpromise guarantee covers the use of ab31287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 48, 75 kDa.

In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies. The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific.We call for caution when this product is used in other assays than Western blot.

IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in neuronal migration during development of the cerebral neocortex. May regulate the stability and proteasomal degradation of the estrogen receptors that play an important role in neuronal differentiation, survival and plasticity.
  • 组织特异性
    Expressed in several tissues, including brain, lung, kidney and testis. In brain localizes to a fraction of cortical neurons and white matter glial cells.
  • 疾病相关
    Defects in DYX1C1 may be a cause of susceptibility to dyslexia type 1 (DYX1) [MIM:127700]. A relatively common, complex cognitive disorder characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities. It is a multifactorial trait, with evidence for familial clustering and heritability. Note=A chromosomal aberration involving DYX1C1 has been found in a family affected by dyslexia. Translocation t(2;15)(q11;q21).
  • 序列相似性
    Contains 1 CS domain.
    Contains 3 TPR repeats.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dyslexia susceptibility 1 candidate 1 antibody
    • Dyslexia susceptibility 1 candidate gene 1 protein antibody
    • DYX1 antibody
    • DYX1C1 antibody
    • DYXC1 antibody
    • DYXC1_HUMAN antibody
    • EKN1 antibody
    • FLJ37882 antibody
    • MGC70618 antibody
    • RD antibody
    see all

图片

  • ab31287 (5μg/ml) staining DYX1C1 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate

    buffer pH 6 and AP-staining shows cytoplasmic staining in some neuronal cells.

  • All lanes : Anti-DYX1C1 antibody (ab31287) at 0.1 µg/ml

    Lane 1 : COS1 cell lysates untransfected
    Lane 2 : COS1 cell lysates transfected with full length recombinant Human DYX1C1


    Observed band size : 48,75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa (possible non-specific binding).

文献

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