RabMAb

Anti-DYNLL1抗体[EP1660Y] (ab51603)

概述

  • 产品名称Anti-DYNLL1抗体[EP1660Y]
    参阅全部 DYNLL1 一抗
  • 描述
    兔单克隆抗体[EP1660Y] to DYNLL1
  • 特异性ab51603 recognizes DLC8.
  • 经测试应用适用于: WB, IHC-P, IP, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to residues on the N terminal of human DLC8.

  • 表位The epitope for this antibody is on the N-terminus, AA2-14.
  • 阳性对照
    • WB: HeLa cell lysate IHC-P: Human liver.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-DYNLL1 antibody (Alexa Fluor® 488) [EP1660Y] (ab203988)

    Anti-DYNLL1 antibody (HRP) [EP1660Y] (ab204008)

应用

Our Abpromise guarantee covers the use of ab51603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P Use at an assay dependent concentration.
IP 1/100.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.
      Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.
      Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.
      Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the dynein light chain family.
    • 翻译后修饰Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.
    • 细胞定位Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Upon induction of apoptosis translocates together with BCL2L11 to mitochondria.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • 8 kDa dynein light chain antibody
      • 8kDLC antibody
      • Cytoplasmic dynein light polypeptide antibody
      • DLC1 antibody
      • DLC8 antibody
      • DNCL1 antibody
      • DNCLC1 antibody
      • DYL1_HUMAN antibody
      • Dynein , cytoplasmic, light chain 1 antibody
      • Dynein light chain 1 cytoplasmic antibody
      • Dynein light chain 1, cytoplasmic antibody
      • Dynein light chain LC8 type 1 antibody
      • Dynein light chain LC8-type 1 antibody
      • Dynein, cytoplasmic, light polypeptide 1 antibody
      • Dynein, light chain, LC8-type 1 antibody
      • DYNLL1 antibody
      • HDLC1 antibody
      • LC8 antibody
      • LC8a antibody
      • MGC126137 antibody
      • MGC126138 antibody
      • MGC72986 antibody
      • PIN antibody
      • Protein inhibitor of neuronal nitric oxide synthase antibody
      • Protein inhibitor of neuronal NOS antibody
      see all

    Anti-DYNLL1 antibody [EP1660Y] 图像

    • All lanes : Anti-DYNLL1 antibody [EP1660Y] (ab51603) at 1/5000 dilution

      Lane 1 : Primary mouse Mb1-Cre control Eµ-Myc B cell lymphoma (lysate of whole lymphnode)
      Lane 2 : Primary mouse Mb1-Cre Dynll1-conditional knockout Eµ-Myc B cell lymphoma (lysate of whole lymphnode)

      Secondary
      HRP conjugated polyclonal goat IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 10 kDa
      Observed band size : 10 kDa


      Exposure time : 10 minutes

      This image is courtesy of an abreview submitted by Dr. Jörg Heierhorst

      Lymphnodes were dissociated in PBS 2% FBS. Cell suspensions filtered through 70 µm and 40 µm cell strainers, and 300 x g pellets were lysed in modified RIPA buffer (150 mM NaCl, 20 mM Tris pH7.4, 1 mM EDTA, 1 mM EGTA, 10 mM NaF, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 x protein inhibitor cocktail (Sigma)).

      See Abreview

    • Immunohistochemical staining of paraffin embedded human liver using ab51603 (1/100).
    • Anti-DYNLL1 antibody [EP1660Y] (ab51603) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 10 kDa
      Observed band size : 10 kDa
    • ab51603 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.

      See Abreview

    • ab51603 used in IP.
      SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.

    Anti-DYNLL1 antibody [EP1660Y] (ab51603)参考文献

    This product has been referenced in:
    • Wong DM  et al. The Transcription Factor ASCIZ and Its Target DYNLL1 Are Essential for the Development and Expansion of MYC-Driven B Cell Lymphoma. Cell Rep 14:1488-99 (2016). Read more (PubMed: 26832406) »
    • Jayappa KD  et al. Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein. J Virol 89:3497-511 (2015). WB, IP, Flow Cyt ; Human . Read more (PubMed: 25568209) »

    See all 11 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (B cell lymphoma lymphnodes)
    Gel Running Conditions Reduced Denaturing (15% midigel)
    Loading amount 12 µg
    Specification B cell lymphoma lymphnodes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Jörg Heierhorst

    Verified customer

    提交于 Apr 13 2016

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (15% GEL)
    Sample Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
    Specification mouse Embryonic Fibroblasts
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 May 30 2014

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (15% GEL)
    Sample Human Cell lysate - other (H23)
    Specification H23
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 13 2013

    Code de remise : xxxxxx
    Date d’expiration : 30/11/12

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab51603 chez la drosophile. Le code de réduction correspondant est xxxxxxxxx. Ce code de réduc...

    Read More

    Merci de votre intérêt pour ab51603. A ma connaissance, cet anticorps n’a pas encore été testé chez la Drosophile et nous n’offrons pas d’échantillon gratuit à des fins de tests pr&eacut...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Recombinant protein (Recombinant protein cloned into EcoR1)
    Loading amount 0.5 µg
    Specification Recombinant protein cloned into EcoR1
    Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycine Gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 07 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (4-12 % gradient gel, Novex)
    Username

    Abcam user community

    Verified customer

    提交于 Mar 30 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Kidney)
    Specification Kidney
    Fixative Methanol
    Permeabilization Yes - 0.1 % Triton
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 22 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"