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Cytoplasmic full length native protein (purified from chick brain) (Chicken).
Our Abpromise guarantee covers the use of ab6304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot Blot||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|Functional Studies||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
|Inhibition Assay||Use at an assay dependent dilution.|
|Purification||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|IHC-P||1/200. Antigen retrieval is not essential but may optimise staining. (see ref Dombeck DA et al). Antigen retrieval is not essential but may optimise staining.|
ab6304 staining rat neuronal stem cells by ICC/IF. Cells were paraformaldehyde fixed, permeabilised with saponin and blocked with 10% serum for 1 hour at 21°C. The primary antibody was diluted 1/100 with PBS/ 1% FCS and incubated for 18 hours at 4°C. A Cy5® conjugated sheep anti-mouse IgG was used as the secondary. Cytoskeleton overlay (green = Phalloidin-FITC)
Image from Andersson L et al, J Cell Sci 119:2246-57 (2006), Fig 8.
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