Anti-Dynein intermediate chain 1抗体[70.1] (ab6304)

概述

  • 产品名称Anti-Dynein intermediate chain 1抗体[70.1]
    参阅全部 Dynein intermediate chain 1 一抗
  • 描述
    小鼠单克隆抗体[70.1] to Dynein intermediate chain 1
  • 特异性This antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched preparations and with crude cell extracts.
  • 经测试应用适用于: Dot blot, ELISA, Functional Studies, ICC, Inhibition Assay, Purification, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Chicken, Hamster, Human, Xenopus laevis, Fish, Indian Muntjac, Marsupials
    不与反应: Mouse
  • 免疫原

    Cytoplasmic full length native protein (purified from chick brain) (Chicken).

性能

应用

Our Abpromise guarantee covers the use of ab6304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
Inhibition Assay Use at an assay dependent dilution.
Purification Use at an assay dependent dilution.
WB 1/2000.
ICC/IF Use at an assay dependent dilution.
IHC-P 1/200. Antigen retrieval is not essential but may optimise staining. (see ref Dombeck DA et al). Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能Part of the dynein complex of respiratory cilia.
  • 疾病相关Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
    Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera).
  • 序列相似性Belongs to the dynein intermediate chain family.
    Contains 5 WD repeats.
  • 细胞定位Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Axonemal dynein intermediate chain 1 antibody
    • Axonemal dynein intermediate chain 2 antibody
    • CILD 1 antibody
    • CILD1 antibody
    • Cytoplasmic dynein 1 intermediate chain 1 antibody
    • Cytoplasmic dynein 1 intermediate chain 2 antibody
    • Cytoplasmic dynein intermediate chain 1 antibody
    • Cytoplasmic dynein intermediate chain 2 antibody
    • DH IC 1 antibody
    • DH IC 2 antibody
    • DIC1 antibody
    • DNAI 1 antibody
    • DNAI 2 antibody
    • DNAI1 antibody
    • DNAI1_HUMAN antibody
    • DNAI2 antibody
    • DNCI 2 antibody
    • DNCI1 antibody
    • DNCI2 antibody
    • DNCIC 1 antibody
    • DNCIC 2 antibody
    • DNCIC1 antibody
    • DNCIC2 antibody
    • DYNC1I1 antibody
    • DYNC1I2 antibody
    • Dynein axonemal intermediate chain 1 antibody
    • Dynein axonemal intermediate polypeptide 1 antibody
    • Dynein axonemal intermediate polypeptide 2 antibody
    • Dynein cytoplasmic intermediate polypeptide 1 antibody
    • Dynein cytoplasmic intermediate polypeptide 2 antibody
    • Dynein intermediate chain 1 axonemal antibody
    • Dynein intermediate chain 1 cytosolic antibody
    • Dynein intermediate chain 1, axonemal antibody
    • Dynein intermediate chain 2 axonemal antibody
    • Dynein intermediate chain 2 cytosolic antibody
    • Dynein intermediate chain DNAI1 antibody
    • IC74 antibody
    • ICS antibody
    • ICS1 antibody
    • Immotile cilia syndrome 1 antibody
    • MGC26204 antibody
    • PCD antibody
    see all

Anti-Dynein intermediate chain 1 antibody [70.1] 图像

  • ab6304 staining rat neuronal stem cells by ICC/IF.  Cells were paraformaldehyde fixed, permeabilised with saponin and blocked with 10% serum for 1 hour at 21°C.  The primary antibody was diluted 1/100 with PBS/ 1% FCS and incubated for 18 hours at 4°C.  A Cy5® conjugated sheep anti-mouse IgG was used as the secondary.  Cytoskeleton overlay (green = Phalloidin-FITC)

    See Abreview

  • Image from Andersson L et al, J Cell Sci 119:2246-57 (2006), Fig 8.

Anti-Dynein intermediate chain 1 antibody [70.1] (ab6304)参考文献

This product has been referenced in:
  • Cao J  et al. Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein. J Virol 89:2777-91 (2015). Read more (PubMed: 25540360) »
  • Joyal JS  et al. Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis. Nat Med 20:1165-73 (2014). Read more (PubMed: 25216639) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neuronal stem cells)
Specification neuronal stem cells
Fixative Paraformaldehyde
Permeabilization Yes - Saponin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Dr. Dominik Schelshorn

Verified customer

提交于 Jan 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative formalin
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Vlady Pavlova

Verified customer

提交于 Dec 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2007

Thank you for your email. Ab6304 is provided in an unpurified form, ascites, and we cannot guarantee the concentration for unpurified material. If you have any more questions, please contact us again.

Thank you for your reply. I would like to know if your customer has applied antigen retrieval. Although for this product antigen retrieval is not essential but it may increase the staining. Please refer your customer to this publication: Dom...

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Thank you for your enquiry. Unfortunately we do not have any information as to whether ab6304 affects the functionality of dynein in live cells. If you have any more questions, please contact us again.

Congratulations and thank you for letting us know about your recent publication using Abcam produce code ab6304 -- Mouse monoclonal to Dynein (intermediate chain)! Unfortunately, the attached reference did not reach me and I would be most grateful if y...

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The immunogen for this antibody was cytoplasmic dynein purified from chick brain. The antibody identifies the cytoplasmic dynein intermediate chains (triplet of approximately 70 kD) of all vertebrates tested, in immunoblotting with dynein enriched pre...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"