概述

  • 产品名称
    Anti-DYNC2H1抗体
  • 描述
    兔多克隆抗体to DYNC2H1
  • 特异性
    ab122525 shows specific reactivity against DYNC2H1.
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    amino acids 3954-4051 (DVFNQRNKKS IFPYSVSLPQ SCSILDYRAV IEKIPEDDKP SFFGLPANIA RSSQRMISSQ VISQLRILGR SITAGSKFDR EIWSNELSPV LNLWKKLN) of Human DYNC2H1.

  • 阳性对照
    • Human small intestine.

性能

应用

Our Abpromise guarantee covers the use of ab122525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May function as a motor for intraflagellar retrograde transport. Functions in cilia biogenesis. May play a role in transport between endoplasmic reticulum and Golgi or organization of the Golgi in cells.
  • 疾病相关
    Defects in DYNC2H1 are the cause of asphyxiating thoracic dystrophy type 3 (ATD3) [MIM:613091]. ATD3 is an autosomal recessive osteochondrodysplasia which often leads to death in infancy because of a severely constricted thoracic cage and respiratory insufficiency.
    Defects in DYNC2H1 are the cause of short rib-polydactyly syndrome type 3 (SRPS3) [MIM:263510]; also called Verma-Naumoff syndrome. A lethal skeletal dysplasia characterized by markedly short ribs, short limbs, polydactyly, narrow thorax, and multiple anomalies of major organs, including heart, intestines, genitalia, kidney, liver, and pancreas.
  • 序列相似性
    Belongs to the dynein heavy chain family.
  • 细胞定位
    Cytoplasm > cytoskeleton > cilium axoneme. Cell membrane. Cytoplasm. Localizes to the apical cytoplasm (By similarity). According to PubMed:8666668, it localizes to Golgi apparatus, cytoplasmic vesicle and endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytoplasmic dynein 2 heavy chain 1 antibody
    • Cytoplasmic dynein 2 heavy chain antibody
    • DYHC2_HUMAN antibody
    • DYNC2H1 antibody
    • Dynein cytoplasmic heavy chain 2 antibody
    • Dynein heavy chain 11 antibody
    • Dynein heavy chain isotype 1B antibody
    • hDHC11 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab122525 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122525, at a 1/100 dilution, staining DYNC2H1 in paraffin embedded Human small intestine tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Wang H  et al. Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomide. Sci Rep 6:28948 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27375225) »
  • Gholkar AA  et al. Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. Cell Cycle 14:1116-25 (2015). Read more (PubMed: 25830415) »

See all 2 Publications for this product

客户评价及客户问答



These antibodies have not been tested specifically against the other form of dynein, however we have aligned the immunogen sequences against the amino acid sequences of each dynein, and there is no significant similarity to the alternative fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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