概述

  • 产品名称Anti-Dynamitin抗体
    参阅全部 Dynamitin 一抗
  • 描述
    小鼠单克隆抗体to Dynamitin
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: DADTQSKVHQ LYETIQRWSP IASTLPELVQ RLVTIKQLHE QAMQFGQLLT HLDTTQQMIA NSLKDNTTLL TQVQTTMREN LATVEGNFAS IDERMKKLGK , corresponding to amino acids 302-401 of Human Dynamitin

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenoma.

性能

应用

Our Abpromise guarantee covers the use of ab56687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
  • 序列相似性Belongs to the dynactin subunit 2 family.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 50 kD dynein associated polypeptide antibody
    • 50 kDa dynein associated polypeptide antibody
    • 50 kDa dynein-associated polypeptide antibody
    • DCTN-50 antibody
    • DCTN2 antibody
    • DCTN2_HUMAN antibody
    • DCTN50 antibody
    • dynactin 2 (p50) antibody
    • dynactin 2 antibody
    • dynactin complex 50 kD subunit antibody
    • Dynactin complex 50 kDa subunit antibody
    • Dynactin subunit 2 antibody
    • DYNAMITIN antibody
    • p50 dynamitin antibody
    • RBP50 antibody
    see all

Anti-Dynamitin antibody 图像

  • Dynamitin antibody (ab56687) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Anti-Dynamitin antibody (ab56687) at 1/500 dilution (in TBS-Tween + 5% milk for 1 hour at 4°C) + Whole cell lysate of HeLa cells at 20 µg

    Secondary
    An IRDye®800CW-conjugated Goat anti-mouse polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step : 5% Milk for 1 hour at 4°C

    See Abreview

  • ab55687 staining Dynamitin in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% BSA for 1 hour at 24°C. Samples were then incubated with the primary antibody at 1/100 for 1 hour at 24°C. The secondary antibody used was a chicken anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.

    See Abreview

  • Total protein input, Hela cell lysate: 500µg.
    Matrix used for Immunoprecipitation step: Protein A Dynabeads.
    ab56687 used at a 1/1000 dilution, 20µl/mg lysate.
    LYS - Lysates
    BC - Beads + Lysates
    IP - Beads + Ab + Lysates

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56687 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56687, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • IHC image of Dynamitin staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56687, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Dynamitin antibody (ab56687)参考文献

This product has been referenced in:
  • Allan AM  et al. Prenatal alcohol exposure modifies glucocorticoid receptor subcellular distribution in the medial prefrontal cortex and impairs frontal cortex-dependent learning. PLoS One 9:e96200 (2014). WB ; Mouse . Read more (PubMed: 24755652) »
  • Wang H  et al. Rotational motion during three-dimensional morphogenesis of mammary epithelial acini relates to laminin matrix assembly. Proc Natl Acad Sci U S A 110:163-8 (2013). Read more (PubMed: 23248267) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - other (HELA)
Total protein in input 500 µg
Specification HELA
Immuno-precipitation step Other - Protein A Dynabeads
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提交于 Jan 24 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TRITON-X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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提交于 Jan 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 50 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HELA)
Loading amount 50 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa Cells)
Loading amount 20 µg
Specification HeLa Cells
Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"