概述

  • 产品名称Anti-Dynamitin抗体
    参阅全部 Dynamitin 一抗
  • 描述
    兔多克隆抗体to Dynamitin
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis , Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Dynamitin (NP_006391.1).

  • 阳性对照
    • Jurkat, 293T, HeLa and NIH3T3 whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab124285 was affinity purified using an epitope specific to Dynamitin immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
  • 序列相似性Belongs to the dynactin subunit 2 family.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 50 kD dynein associated polypeptide antibody
    • 50 kDa dynein associated polypeptide antibody
    • 50 kDa dynein-associated polypeptide antibody
    • DCTN-50 antibody
    • DCTN2 antibody
    • DCTN2_HUMAN antibody
    • DCTN50 antibody
    • dynactin 2 (p50) antibody
    • dynactin 2 antibody
    • dynactin complex 50 kD subunit antibody
    • Dynactin complex 50 kDa subunit antibody
    • Dynactin subunit 2 antibody
    • DYNAMITIN antibody
    • p50 dynamitin antibody
    • RBP50 antibody
    see all

Anti-Dynamitin antibody 图像

  • All lanes : Anti-Dynamitin antibody (ab124285) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Exposure time : 30 seconds
  • ab124285 at 1 µg/ml staining Dynamitin in Jurkat whole cell lysate immunoprecipitated using ab124285 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 44 kDa. Exposure time: 3 seconds

Anti-Dynamitin antibody (ab124285)参考文献

ab124285 has not yet been referenced specifically in any publications.

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