Dynamin Inhibitors: Dynole® Series试剂盒(ab120474)

概述

  • 产品名称Dynamin Inhibitors: Dynole® Series试剂盒
  • 特异性Convenient kit containing dynamin inhibitor Dynole® 34-2 (ab120463; 1 mg) and negative control Dynole® 31-2 (ab120464; 1mg) from the Dynole chemical series. Dynole® 34-2 targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in emzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
  • 产品概述

    DynoleTM chemical series collection

    Convenient kit containing dynamin inhibitor Dynole-34-2™ (ab120463; 1 mg) and negative control Dynole-31-2™ (ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.

    Target

    Description

    GTPase Allosteric Site

    Dynole-34-2™:

    Potent dynamin I and dynamin II inhibitor

    GTPase Allosteric Site

    Dynole-31-2™:

    Negative control for Dynole 34-2™ 

    Convenient kit containing dynamin inhibitor Dynole-34-2™ ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.

  • 说明

    Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab120474 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Dynamin Inhibitors: Dynole® Series Kit 图像

    • ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Dynamin Inhibitors: Dynole® Series Kit (ab120474)参考文献

    This product has been referenced in:
    • Schulze RJ  et al. Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes. J Cell Biol 203:315-26 (2013). Functional Studies . Read more (PubMed: 24145164) »
    • Hill TA  et al. Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles. J Med Chem 52:3762-73 (2009). Read more (PubMed: 19459681) »

    See all 2 Publications for this product

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