概述

  • 产品名称Anti-Dynamin 2抗体
    参阅全部 Dynamin 2 一抗
  • 描述
    兔多克隆抗体to Dynamin 2
  • 经测试应用适用于: IP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Dynamin 2.

    (Peptide available as ab71849.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Testis Tissue Ramos Whole Cell Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM) HeLa Whole Cell K562 Whole Cell Rat Thymus Tissue SK N BE Whole Cell

性能

应用

Our Abpromise guarantee covers the use of ab65556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 98 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性Belongs to the dynamin family.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMT2M antibody
    • CMTDI1 antibody
    • CMTDIB antibody
    • DI CMTB antibody
    • Dnm2 antibody
    • DYN II antibody
    • DYN2 antibody
    • DYN2_HUMAN antibody
    • Dynamin II antibody
    • Dynamin-2 antibody
    • Dynamin2 antibody
    • DynaminII antibody
    • DYNII antibody
    see all

Anti-Dynamin 2 antibody 图像

  • All lanes : Anti-Dynamin 2 antibody (ab65556) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 6 : Rat Thymus Tissue Lysate
    Lane 7 : SK N BE (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 98 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Dynamin 2 was immunoprecipitated using 0.5mg Rat Testis whole tissue extract, 5µg of Rabbit polyclonal to Dynamin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Testis whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65556.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 100kDa: Dynamin 2.
  • ICC/IF image of ab65556 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65556, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 1µg/ml.
  • ab65556 (1/250) staining Dynamin 2 in paraffin-embedded Human lung tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.

    See Abreview

Anti-Dynamin 2 antibody (ab65556)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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提交于 Nov 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"