概述

  • 产品名称
    Anti-Dynamin 1 (phospho S774)抗体
    参阅全部 Dynamin 1 一抗
  • 描述
    兔多克隆抗体to Dynamin 1 (phospho S774)
  • 特异性
    This antibody detects endogenous levels of Dynamin 1 only when phosphorylated at serine 774.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human Dynamin 1 around the phosphorylation site of serine 774 (R-R-SP-P-T).

  • 阳性对照
    • Mouse brain extracts.

性能

应用

Our Abpromise guarantee covers the use of ab55324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted mouse molecular weight: 98 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • All lanes : Anti-Dynamin 1 (phospho S774) antibody (ab55324) at 1/500 dilution

      Lane 1 : Mouse brain extract
      Lane 2 : Mouse brain extract treated with the immunising phosphopeptide.


      Predicted band size : 98 kDa
      Observed band size : 98 kDa

    实验方案

    文献

    This product has been referenced in:
    • Reis CR  et al. Crosstalk between Akt/GSK3ß signaling and dynamin-1 regulates clathrin-mediated endocytosis. EMBO J 34:2132-46 (2015). Read more (PubMed: 26139537) »
    • Mori H  et al. Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1. Development 140:343-52 (2013). WB ; Mouse . Read more (PubMed: 23250208) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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