概述

  • 产品名称Anti-Dynamin 1抗体[D5]
    参阅全部 Dynamin 1 一抗
  • 描述
    小鼠单克隆抗体[D5] to Dynamin 1
  • 经测试应用适用于: IP, Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Cow, Human
  • 免疫原

    Recombinant His-tagged fusion protein, corresponding to amino acids 1-750 of Human Dynamin 1.

  • 阳性对照
    • This antibody gave a positive signal in Mouse and Rat Brain Tissue Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab13251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
IHC-P 1/50.

靶标

Anti-Dynamin 1 antibody [D5] 图像

  • All lanes : Anti-Dynamin 1 antibody [D5] (ab13251) at 5 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 104 kDa


    Exposure time : 1 minute
  • Dynamin 1 was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Dynamin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13251.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
    Band: 104kDa; Dynamin 1

Anti-Dynamin 1 antibody [D5] (ab13251)参考文献

This product has been referenced in:
  • Ku YC  et al. The transcriptome of utricle hair cell regeneration in the avian inner ear. J Neurosci 34:3523-35 (2014). Read more (PubMed: 24599453) »
  • Nath S  et al. A huntingtin-mediated fast stress response halting endosomal trafficking is defective in Huntington's disease. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 25205111) »

See all 4 Publications for this product

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