概述

  • 产品名称Anti-Dux4抗体[E5-5]
    参阅全部 Dux4 一抗
  • 描述
    兔单克隆抗体[E5-5] to Dux4
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Dux4.

  • 阳性对照
    • WB: Human, mouse and rat testis and mouse and rat brain tissue lysates. HT 1080, PC 3, and HeLa cells lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号E5-5
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 相关性DUX4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in DUX4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Severity is highly variable. Weakness is slowly progressive and about 20% of affected individuals eventually require a wheelchair. Approximately 70-90% of individuals have inherited the disease-causing deletion from a parent, and approximately 10-30% of affected individuals have FSHD as the result of a de novo deletion. Offsprings of an affected individual have a 50% chance of inheriting the deletion.
    • 细胞定位Nuclear
    • 数据库链接
    • 别名
      • Double homeobox protein 10 antibody
      • Double homeobox protein 4 antibody
      • Double homeobox protein 4/10 antibody
      • DUX10 antibody
      • DUX4L1 antibody
      see all

    Anti-Dux4 antibody [E5-5] 图像

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (purified)

      Lane 1 : HT-1080 whole cell lysate
      Lane 2 : PC-3 whole cell lysate
      Lane 3 : Saos-2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse testis tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa


      Exposure time : 3 minutes
    • All lanes : Anti-Dux4 antibody [E5-5] (ab124699) at 1/1000 dilution (unpurified)

      Lane 1 : Human testis lysate
      Lane 2 : HT 1080 cell lysate
      Lane 3 : PC 3 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 45 kDa

    Anti-Dux4 antibody [E5-5] (ab124699)参考文献

    This product has been referenced in:
    • Ferri G  et al. Direct interplay between two candidate genes in FSHD muscular dystrophy. Hum Mol Genet 24:1256-66 (2015). ChIP ; Human . Read more (PubMed: 25326393) »
    • Himeda CL  et al. Myogenic enhancers regulate expression of the facioscapulohumeral muscular dystrophy-associated DUX4 gene. Mol Cell Biol 34:1942-55 (2014). ICC/IF . Read more (PubMed: 24636994) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Rhabdomyosarcoma cell line)
    Specification Rhabdomyosarcoma cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X-100 in PBS
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"