概述

  • 产品名称Anti-DUT抗体
    参阅全部 DUT 一抗
  • 描述
    兔多克隆抗体to DUT
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence mapping at the middle region of human DUT-N, identical to the related rat and mouse sequence.

  • 阳性对照
    • Rat cardiac muscle tissue lysate and rat cardiac muscle tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab65971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 1 - 2 µg/ml. Boiling the sections is required.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: ~23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • 组织特异性Found in a variety of tissues.
    • 通路Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • 序列相似性Belongs to the dUTPase family.
    • 翻译后修饰Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • 细胞定位Mitochondrion and Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody 图像

    • ab65971 at 1µg/ml staining DUT-N in the cytoplasm of paraffin-embedded rat tissue sections (cardiac muscle). (DAB chromogenic reaction)
    • Anti-DUT antibody (ab65971) at 0.5 µg/ml + Rat cardiac muscle tissue lysate

      Predicted band size : 23 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    Anti-DUT antibody (ab65971)参考文献

    This product has been referenced in:
    • Luo Y  et al. Uracil incorporation into genomic DNA does not predict toxicity caused by chemotherapeutic inhibition of thymidylate synthase. DNA Repair (Amst) 7:162-9 (2008). Read more (PubMed: 17942376) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"