概述

  • 产品名称Anti-DUT抗体
    参阅全部 DUT 一抗
  • 描述
    兔多克隆抗体to DUT
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 25-44 (NARGTAEGRSRGTLRARPAP) and 113-130 (QLRFARLSEHATAPTRGS) of Human DUT-N.

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Please note:
    Quality control data for this antibody: 20 ug of HeLa cell lysate was used in one lane of a mini blot. At a 1 min exposure, two bands are seen (as shown on the datasheet): a predominant band at 21 kDa and a lighter band at ~31 kDa.
    At a 15 min exposure, a 90 kDa band is also observed, along with much lighter bands at 66 and 55 kDa. Therefore please note that additional bands can appear with prolonged exposure, high antibody concentrations or high lysate concentrations, this may mask the specific band.
  • 靶标

    • 功能This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
    • 组织特异性Found in a variety of tissues.
    • 通路Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
    • 序列相似性Belongs to the dUTPase family.
    • 翻译后修饰Phosphorylation in mature T-cells occur in a cell cycle-dependent manner.
    • 细胞定位Mitochondrion and Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody
      • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody
      • Deoxyuridine triphosphatase antibody
      • dut antibody
      • DUT_HUMAN antibody
      • dUTP nucleotidohydrolase antibody
      • dUTP pyrophosphatase antibody
      • dUTPase antibody
      • FLJ20622 antibody
      see all

    Anti-DUT antibody 图像



    • Predicted band size : 27 kDa


      Western blot detection of DUT-N in HeLa cell lysate using ab13690 at a dilution of 1:1,000.

    Anti-DUT antibody (ab13690)参考文献

    This product has been referenced in:
    • Luo Y  et al. Uracil incorporation into genomic DNA does not predict toxicity caused by chemotherapeutic inhibition of thymidylate synthase. DNA Repair (Amst) 7:162-9 (2008). WB ; Human . Read more (PubMed: 17942376) »
    • Wu M  et al. dUTP Pyrophosphatase, its appearance in extracellular compartment may serve as a potential biomarker for N-methyl-N'-nitro-N-nitrosoguanidine exposure in mammalian cells. Proteomics 6:3001-7 (2006). WB ; Human . Read more (PubMed: 16619294) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"