概述

  • 产品名称
  • 描述
    兔多克隆抗体to DUSP7
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide with a sequence corresponding to a region within amino acids 258 and 320 of Human DUSP7 (NP_001938)

  • 阳性对照
    • 293T, H1299, HepG2, Molt-4 and Raji cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab95960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Regulates the activity of the MAP kinase family in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L although it is unclear whether this is by competing for transcription, translation or activation factors.
  • 组织特异性
    Expressed at significantly higher levels in malignant hematopoietic cells than in corresponding non-malignant cells.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity phosphatase 7 antibody
    • Dual specificity protein phosphatase 7 antibody
    • Dual specificity protein phosphatase PYST2 antibody
    • DUS7_HUMAN antibody
    • Dusp7 antibody
    • Jnk Stimulatory Phosphatase 1 antibody
    • JSP1 antibody
    • MAP kinase phosphatase X antibody
    • MKPX antibody
    • PYST2 antibody
    see all

图片

  • Anti-DUSP7 antibody (ab95960) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 41 kDa



    10% SDS PAGE
  • ab95960, at 1/200, staining paraformaldehyde-fixed A549 cells by Immunofluorescence. Lower image merged with DNA probe.
  • ab95960, at 1/100, staining paraffin-embedded NCIN87 xenograft, by Immunohistochemistry.

文献

ab95960 has not yet been referenced specifically in any publications.

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary MEF)
Loading amount
5 µg
Specification
Primary MEF
Treatment
100ngµl TPA for 2 hours
Gel Running Conditions
Reduced Denaturing (4-14% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册