概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to DUSP6
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-382 of Human DUSP6a

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Inactivates MAP kinases. Has a specificity for the ERK family.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity phosphatase 6 antibody
    • Dual specificity phosphatase 6 isoform a antibody
    • Dual specificity protein phosphatase 6 antibody
    • Dual specificity protein phosphatase PYST1 antibody
    • DUS6_HUMAN antibody
    • DUSP 6 antibody
    • DUSP 6a antibody
    • Dusp6 antibody
    • DUSP6a antibody
    • HH19 antibody
    • MAP kinase phosphatase 3 antibody
    • Mitogen activated protein kinase phosphatase 3 antibody
    • Mitogen-activated protein kinase phosphatase 3 antibody
    • MKP 3 antibody
    • MKP-3 antibody
    • MKP3 antibody
    • PYST 1 antibody
    • PYST1 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

图片

  • DUSP6a antibody (ab54940) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.


  • Predicted band size : 42 kDa
    DUSP6a antibody (ab54940) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • ab54940 at 10 ug/ml staining DUSP6 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing SH-SY5Y cells stained with ab54940 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54940, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Wittig-Blaich S  et al. Systematic screening of isogenic cancer cells identifies DUSP6 as context-specific synthetic lethal target in melanoma. Oncotarget 8:23760-23774 (2017). WB ; Human . Read more (PubMed: 28423600) »
  • Ackley A  et al. An Algorithm for Generating Small RNAs Capable of Epigenetically Modulating Transcriptional Gene Silencing and Activation in Human Cells. Mol Ther Nucleic Acids 2:e104 (2013). WB ; Human . Read more (PubMed: 23839098) »

See all 4 Publications for this product

客户评价及客户问答

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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