概述

  • 产品名称Anti-DUSP5抗体
    参阅全部 DUSP5 一抗
  • 描述
    小鼠单克隆抗体to DUSP5
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: LKEAFDYIKQ RRSMVSPNFG FMGQLLQYES EILPSTPNPQ PPSCQGEAAG SSLIGHLQTL SPDMQGAYCT FPASVLAPVP THSTVSELSR SPVATATSC, corresponding to amino acids 286-385 of Human DUSP5

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity phosphatase 5 antibody
    • Dual specificity protein phosphatase 5 antibody
    • Dual specificity protein phosphatase hVH 3 antibody
    • Dual specificity protein phosphatase hVH3 antibody
    • DUS5_HUMAN antibody
    • DUSP 5 antibody
    • DUSP antibody
    • DUSP5 antibody
    • HVH 3 antibody
    • HVH3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH 3 antibody
    • VH1 like phosphatase 3 antibody
    • VH3 antibody
    see all

Anti-DUSP5 antibody 图像



  • Predicted band size : 42 kDa
    DUSP5 antibody (ab54939) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54939 at 10 ug/ml staining DUSP5 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing SH-SY5Y cells stained with ab54939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-DUSP5 antibody (ab54939)参考文献

This product has been referenced in:
  • Wickramasekera NT  et al. Role of dual-specificity protein phosphatase-5 in modulating the myogenic response in rat cerebral arteries. J Appl Physiol 114:252-61 (2013). Read more (PubMed: 23172031) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (Hela and K562)
Specification Hela and K562
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (NSCLC lung cancer cell - A549)
Loading amount 100 µg
Specification NSCLC lung cancer cell - A549
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"