概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to DUSP5
  • 经测试应用
    适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: LKEAFDYIKQ RRSMVSPNFG FMGQLLQYES EILPSTPNPQ PPSCQGEAAG SSLIGHLQTL SPDMQGAYCT FPASVLAPVP THSTVSELSR SPVATATSC, corresponding to amino acids 286-385 of Human DUSP5

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Displays phosphatase activity toward several substrates. The highest relative activity is toward ERK1.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity phosphatase 5 antibody
    • Dual specificity protein phosphatase 5 antibody
    • Dual specificity protein phosphatase hVH 3 antibody
    • Dual specificity protein phosphatase hVH3 antibody
    • DUS5_HUMAN antibody
    • DUSP 5 antibody
    • DUSP antibody
    • DUSP5 antibody
    • HVH 3 antibody
    • HVH3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH 3 antibody
    • VH1 like phosphatase 3 antibody
    • VH3 antibody
    see all

图片



  • Predicted band size : 42 kDa
    DUSP5 antibody (ab54939) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54939 at 10 ug/ml staining DUSP5 in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Overlay histogram showing SH-SY5Y cells stained with ab54939 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

文献

This product has been referenced in:
  • Wang J  et al. Long Non-coding RNA H19 Induces Cerebral Ischemia Reperfusion Injury via Activation of Autophagy. Aging Dis 8:71-84 (2017). WB . Read more (PubMed: 28203482) »
  • Wickramasekera NT  et al. Role of dual-specificity protein phosphatase-5 in modulating the myogenic response in rat cerebral arteries. J Appl Physiol 114:252-61 (2013). Read more (PubMed: 23172031) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Hela and K562)
Specification
Hela and K562
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (NSCLC lung cancer cell - A549)
Loading amount
100 µg
Specification
NSCLC lung cancer cell - A549
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册