Anti-DUSP27抗体(ab121372)

概述

  • 产品名称Anti-DUSP27抗体
  • 描述
    兔多克隆抗体to DUSP27
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 250-282 (EALMTVRKKR AIYPNEGFLK QLRELNEKLM EER) of Human DUSP27 (Q5VZP5).

  • 阳性对照
    • Human testis tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab121372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-DUSP27 antibody 图像

  • ab121372 at 1/100 dilution staining DUSP27 in Paraffin-embedded Human Testis tissue by Immunohistochemistry.

Anti-DUSP27 antibody (ab121372)参考文献

ab121372 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 60 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Treatment siRNA transfected
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Mouse Cell lysate - whole cell (Kidney)
Specification Kidney
Treatment plasmid transfected
Total protein in input 500 µg
Username

Abcam user community

Verified customer

提交于 Sep 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Sample Mouse Cell (mouse muscle C2C12)
Specification mouse muscle C2C12
Permeabilization Yes - 0.3% triton in PBS
Fixative Paraformaldehyde
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Yanfeng

Verified customer

提交于 Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"