ab70124 detects endogenous levels of total DUSP22 protein.
Synthetic peptide derived from an internal sequence of human DUSP22.
Jurkat and RAW264.7 cell extracts.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab70124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).
Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Information by UniProt
Dual specificity protein phosphatase 22 antibody
Western blot - DUSP22 antibody (ab70124)
All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution Lane 1 : Jurkat cell extracts Lane 2 : RAW264.7 cell extracts Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 20 kDa Observed band size: 20 kDa
Immunocytochemistry/ Immunofluorescence-DUSP22 antibody(ab70124)
ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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