Anti-DUSP16抗体(ab65151)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to DUSP16
  • 特异性
    ab65151 detects endogenous levels of total DUSP16 protein.
  • 经测试应用
    适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human DUSP16.

  • 阳性对照
    • Jurkat and COLO205 cell extracts and HepG2 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab65151 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

靶标

  • 功能
    Involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • 细胞定位
    Cytoplasm. Nucleus. Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity phosphatase 16 antibody
    • Dual specificity protein phosphatase 16 antibody
    • DUS16_HUMAN antibody
    • DUSP 16 antibody
    • DUSP16 antibody
    • KIAA1700 antibody
    • MAP kinase phosphatase 7 antibody
    • MAPK phosphatase 7 antibody
    • Mitogen activated protein kinase phosphatase 7 antibody
    • Mitogen-activated protein kinase phosphatase 7 antibody
    • MKP 7 antibody
    • MKP-7 antibody
    • MKP7 antibody
    see all

Anti-DUSP16 antibody 图像

  • ab65151 at 1/500 dilution staining DUSP16 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-DUSP16 antibody (ab65151) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO205 cell extract
    Lane 3 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

Anti-DUSP16 antibody (ab65151)参考文献

ab65151 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine embryonic fibroblasts)
Loading amount
15 µg
Specification
murine embryonic fibroblasts
Treatment
300µM hydrogen peroxide, 0.5h & 4h
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 May 01 2013

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