概述

性能

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应用

Our Abpromise guarantee covers the use of ab1351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Most of the commercially available BSA and dry milk are contaminated with bovine IgG (even some of the higher purity grades of BSA); this might cause high background problems when labelled anti-goat secondary is used. The secondary might cross-react with bovine IgG since goat is closely related to cow.

靶标

  • 功能Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
  • 组织特异性Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CL 100 antibody
    • CL100 antibody
    • Dual Specificity Phosphatase 1 antibody
    • Dual specificity protein phosphatase 1 antibody
    • Dual specificity protein phosphatase hVH1 antibody
    • DUS1_HUMAN antibody
    • DUSP 1 antibody
    • Dusp1 antibody
    • HVH1 antibody
    • MAP kinase phosphatase 1 antibody
    • Mitogen-activated protein kinase phosphatase 1 antibody
    • MKP-1 antibody
    • MKP1 antibody
    • Protein tyrosine phosphatase CL100 antibody
    • Protein-tyrosine phosphatase CL100 antibody
    • PTPN10 antibody
    • Serine/threonine specific protein phosphatase antibody
    • VH1 antibody
    see all

Anti-DUSP1 antibody 图像

  • Endogenous DUSP1 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
  • ab1351 (3.8 µg/ml) staining DUSP1 in paraffin-embedded Human prostate tissue. Steam antigen retrieval was performed with citrate buffer (pH 6).

Anti-DUSP1 antibody (ab1351)参考文献

This product has been referenced in:
  • Delehouzé C  et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 24317512) »
  • Giachini FR  et al. Extracellular signal-regulated kinase 1/2 activation, via downregulation of mitogen-activated protein kinase phosphatase 1, mediates sex differences in desoxycorticosterone acetate-salt hypertension vascular reactivity. Hypertension 55:172-9 (2010). WB ; Rat . Read more (PubMed: 19901158) »

See all 3 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Chicken Cell lysate - whole cell (Thrombocyte)
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Loading amount 2 µg
Specification Thrombocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mrs. Katie Elliott

Verified customer

提交于 Sep 29 2016

Application Immunocytochemistry
Sample Human Cultured Cells (Glioblastoma cells (U87MG, LN18,..) (all human))
Permeabilization Yes - Triton x
Specification Glioblastoma cells (U87MG, LN18,..) (all human)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Matthias Dedobbeleer

Verified customer

提交于 May 04 2016

We recommend using a permeablization step with this product in IHC-P. Permeabilize with 0.25% Triton X-100 for 10 minutes at room temperature. Perform this step after antigen retrieval and before blocking.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (spinal cord homogenate)
Loading amount 20 µg
Specification spinal cord homogenate
Treatment sham or nerve transected
Gel Running Conditions Reduced Denaturing (10% SDS_PAGE)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 17 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (HAPI cell line)
Loading amount 400000 cells
Specification HAPI cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (BV2 cell line)
Loading amount 400000 cells
Specification BV2 cell line
Treatment LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Primary Microglia)
Loading amount 400000 cells
Specification Primary Microglia
Treatment LPS 50 ng/mL or LPS 50 ng/mL +UO 10 uM
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2008

Thank you for your enquiry. I apologize for the delay in answering your question due to the holidays. The protocol used to test this antibody is below. 35ug protein per lane was loaded and its concentration would have been 2.5mg/ml in SDS Page loa...

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Thank you for your enquiry. The Western on the datasheet is detecting endogenous DUSP1 in Hela. In order to further troubleshoot why this antibody is not giving you any signal, it would be helpful if you could fill out the form at the link I am i...

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Thank you for your enquiry and for your patience. The originator of this antibody has informed me that they only observed a single band in HeLa lysate and we have not had any other feedback yet regarding the use of ab1351. However, looking at the seque...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"