概述

  • 产品名称Anti-DSN1抗体
    参阅全部 DSN1 一抗
  • 描述
    小鼠单克隆抗体to DSN1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment: MTYLGSSQNE VLNTKPDYQK ILQNQSKVFD CMELVMDELQ GSVKQLQAFM DESTQCFQKV SVQLGKRSMQ QLDPSPARKL LKLQLQNPPA IHGSGSGSCQ, corresponding to amino acids 257-356 of Human DSN1 (NP_079194) with a 26 kDa tag.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
    • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C20orf172 antibody
      • Chromosome 20 open reading frame 172 antibody
      • dJ469A13.2 antibody
      • DSN1 antibody
      • DSN1, MIND kinetochore complex component, homolog antibody
      • DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
      • DSN1, MIS12 kinetochore complex component antibody
      • DSN1_HUMAN antibody
      • FLJ13346 antibody
      • hKNL-3 antibody
      • Kinetochore associated protein DSN1 antibody
      • Kinetochore associated protein DSN1 homolog antibody
      • Kinetochore null 3 homolog antibody
      • Kinetochore-associated protein DSN1 homolog antibody
      • KNL3 antibody
      • MGC32987 antibody
      • MIS13 antibody
      see all

    Anti-DSN1 antibody 图像

    • Anti-DSN1 antibody (ab77791) at 1/500 dilution + recombinant fragment (the immunogen) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 40 kDa
      Observed band size : 40 kDa

    Anti-DSN1 antibody (ab77791)参考文献

    ab77791 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Aug 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"