概述

  • 产品名称
    Anti-Dsk2抗体
  • 描述
    兔多克隆抗体to Dsk2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein (S. cerevisiae). Fusion protein Dsk2 expressed in E. coli was purified and thrombin-digested full-length Dsk2 was used for antigen.

性能

应用

Our Abpromise guarantee covers the use of ab4119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 51 kDa.

靶标

  • 相关性
    Dsk2p is a member of a protein family that is characterized by possession of an N-terminal ubiquitin (Ub)-like (UbL) domain and a C-terminal Ub-associated (UBA) domain. In yeast, DSK2 was isolated as a suppressor of kar1, which is defective in spindle pole duplication. Although DSK2 is not an essential gene, yeast strains deleted for both DSK2 and RAD23 are temperature-sensitive for growth because of a block of spindle pole body duplication. Dsk2p and Rad23p seems to perform overlapping functions, although the sequence of Dsk2p is hardly similar to that of Rad23p overall except that they show 30% identity in their N-terminal UbL domains (residues 1-76) and that both contain UBA domains at their C terminus. Recent work on yeast Rad23p indicates that it is connected with the Ub-proteasome pathway; the N-terminal UbL domain interacts with the proteasome. There are genetic interactions between RAD23 and the polyubiquitin-binding subunit of the proteasome, RPN10, and Rad23p seems to inhibit polyubiquitin chain assembly in a cell-free assay system. It has recently been identified that Xenopus Dsk2-related protein, XDRP1, blocked Ub-dependent degradation of the cyclin A, and the human homologue of XDRP1, Plic1, is reported to form a link between the Ub machinery and the proteasome. Plic1 also is known as ubiquilin, independently isolated in a yeast two-hybrid screen by using presenilin as bait.
  • 细胞定位
    Nuclear
  • 数据库链接
    • 别名
      • SHE4 antibody
      • Ubiquitin domain containing protein DSK2 antibody
      • YMR276W antibody

    图片

    • Lane 1: Yeast extract from dsk2 knockout strain.
      Lane 2: Yeast extract from DSK2 strain.

    实验方案

    文献

    This product has been referenced in:
    • Pfingsten JS  et al. Mutually exclusive binding of telomerase RNA and DNA by Ku alters telomerase recruitment model. Cell 148:922-32 (2012). Read more (PubMed: 22365814) »
    • Funakoshi M  et al. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A 99:745-50 (2002). Read more (PubMed: 11805328) »

    See all 2 Publications for this product

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