antigen sequence, corresponding to amino acids 82-155 (GKFPSGKTEI PFEFPLHLKG NKVLYETYHG VFVNIQYTLR CDMKRSLLAK DLTKTCEFIV HSAPQKGKFT PSPV) of Human DSCR3.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-DSCR3 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-DSCR3 antibody (ab121747)
Immunofluorescent staining of Human cell line U-251MG shows positivity in mitochondria. Recommended concentration of ab121747 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DSCR3 antibody (ab121747)
ab121747 at 1/400 dilution staining DSCR3 in Paraffin-emebedded Human rectum tissue by Immunohistochemistry.
Anti-DSCR3 antibody (ab121747)参考文献
has not yet been referenced specifically in any publications.
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