Anti-DSCC1抗体(ab106972)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to DSCC1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 318-347 of Human DSCC1 (NP_076999.2).

  • 阳性对照
    • CEM cell line lysate

性能

应用

Our Abpromise guarantee covers the use of ab106972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
ICC/IF 1/200. Use with paraformaldehyde-fixed cells.

靶标

  • 功能
    Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.
  • 序列相似性
    Belongs to the DCC1 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCC1 antibody
    • DCC1_HUMAN antibody
    • Defective in sister chromatid cohesion 1 homolog antibody
    • Defective in sister chromatid cohesion protein 1 homolog antibody
    • DSCC1 antibody
    • hDCC1 antibody
    • MGC5528 antibody
    • Sister chromatid cohesion protein DCC1 antibody
    see all

图片

  • Anti-DSCC1 antibody (ab106972) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 45 kDa
  • ab106972 (1/200) staining DSCC1 in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please refer to Abreview.

    See Abreview

文献

ab106972 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
90 µg
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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