概述

  • 产品名称Anti-Dscam抗体
    参阅全部 Dscam 一抗
  • 描述
    兔多克隆抗体to Dscam
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Fruit fly (Drosophila melanogaster) Dscam.

    (Peptide available as ab43846.)

  • 阳性对照
    • This antibody gave a positive signal in Drosophila S2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa.

靶标

  • 功能Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
  • 组织特异性Primarily expressed in brain.
  • 序列相似性Contains 6 fibronectin type-III domains.
    Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
  • 翻译后修饰Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
  • 细胞定位Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CHD2 42 antibody
      • CHD2 52 antibody
      • CHD2 antibody
      • Down syndrome cell adhesion molecule antibody
      • DSCAM antibody
      • DSCAM_HUMAN antibody
      • human CHD2-52 down syndrome cell adhesion molecule 2, 9 antibody
      • OTTHUMP00000109193 2 antibody
      • SD11109p antibody
      see all

    Anti-Dscam antibody 图像

    • Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 222 kDa
      Observed band size : 235 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

    Anti-Dscam antibody (ab43847)参考文献

    This product has been referenced in:
    • Jin XK  et al. Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis. Fish Shellfish Immunol 35:900-9 (2013). Read more (PubMed: 23856639) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heads and embryos)
    Loading amount 10 µg
    Specification fly heads and embryos
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
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    提交于 Apr 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"