Anti-ds DNA抗体[35I9 DNA] - BSA and Azide free (ab27156)
Key features and details
- Mouse monoclonal [35I9 DNA] to ds DNA - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-ds DNA抗体[35I9 DNA] - BSA and Azide free
参阅全部 ds DNA 一抗 -
描述
小鼠单克隆抗体[35I9 DNA] to ds DNA - BSA and Azide free -
宿主
Mouse -
特异性
Primarily Double stranded DNA. Measurements by immuno-CE yielded KD's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
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阳性对照
- IHC-P: human skin tissue sections.
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常规说明
This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113). Or search our wide range of secondary antibodies for use with your experiment.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength. -
克隆
单克隆 -
克隆编号
35I9 DNA -
骨髓瘤
x63-Ag8.653 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27156于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs. -
细胞定位
Nuclear -
别名
- Double stranded deoxyribonucleic acid antibody
- Double stranded DNA antibody
- dsDNA antibody
图片
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IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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Datasheet download
文献 (99)
ab27156 被引用在 99 文献中.
- Karam J et al. Rapid communication: insights into the role of extracellular vesicles during Auger radioimmunotherapy. Int J Radiat Biol 99:109-118 (2023). PubMed: 34270378
- Shen H et al. Synergistically targeting synovium STING pathway for rheumatoid arthritis treatment. Bioact Mater 24:37-53 (2023). PubMed: 36582350
- Chang CH et al. The first reported case of trastuzumab induced interstitial lung disease associated with anti-neutrophil cytoplasmic antibody vasculitis - A case report and a prospective cohort study on the usefulness of neutrophil derived biomarkers in monitoring vasculitis disease activity during follow-up. Breast 61:35-42 (2022). PubMed: 34894465
- Yan Q et al. Proximity labeling identifies a repertoire of site-specific R-loop modulators. Nat Commun 13:53 (2022). PubMed: 35013239
- Neufeldt CJ et al. SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB. Commun Biol 5:45 (2022). PubMed: 35022513