Anti-DRP1抗体[OTI4F6] (ab156951)
Key features and details
- Mouse monoclonal [OTI4F6] to DRP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG2b
概述
-
产品名称
Anti-DRP1抗体[OTI4F6]
参阅全部 DRP1 一抗 -
描述
小鼠单克隆抗体[OTI4F6] to DRP1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein, corresponding to amino acids 1-736 of Human DRP1 produced in HEK293T cells (NP_036192).
-
阳性对照
- HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cell extracts; Human lung carcinoma, ovary adenocarcinoma, pancreas, pancreas carcinoma and endometrium tissues.
-
常规说明
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 4F6 to OTI4F6, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
OTI4F6 -
同种型
IgG2b -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156951于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/200 - 1/4000. Predicted molecular weight: 82 kDa.
|
|
IHC-P |
1/150.
|
说明 |
---|
WB
1/200 - 1/4000. Predicted molecular weight: 82 kDa. |
IHC-P
1/150. |
靶标
-
功能
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed. -
组织特异性
Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. -
疾病相关
Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage. -
序列相似性
Belongs to the dynamin family.
Contains 1 GED domain. -
结构域
The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization. -
翻译后修饰
Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
Ubiquitination by MARCH5 affects mitochondrial morphology. -
细胞定位
Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. - Information by UniProt
-
数据库链接
- Entrez Gene: 10059 Human
- Entrez Gene: 74006 Mouse
- Entrez Gene: 114114 Rat
- Omim: 603850 Human
- SwissProt: O00429 Human
- SwissProt: Q8K1M6 Mouse
- SwissProt: O35303 Rat
- Unigene: 556296 Human
see all -
别名
- DLP1 antibody
- dnm1l antibody
- DNM1L_HUMAN antibody
see all
图片
-
All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418 -
All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 82 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418 -
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
-
Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
-
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DRP1 with ab156951 at 1/150 dilution
-
Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
-
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling DRP1 with ab156951 at 1/150 dilution
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab156951 被引用在 9 文献中.
- Sun L et al. The E3 Ubiquitin Ligase SYVN1 Plays an Antiapoptotic Role in Polycystic Ovary Syndrome by Regulating Mitochondrial Fission. Oxid Med Cell Longev 2022:3639302 (2022). PubMed: 36193086
- Adlimoghaddam A et al. Nilotinib Improves Bioenergetic Profiling in Brain Astroglia in the 3xTg Mouse Model of Alzheimer's Disease. Aging Dis 12:441-465 (2021). PubMed: 33815876
- Antunes MA et al. Mesenchymal Stromal Cells From Emphysematous Donors and Their Extracellular Vesicles Are Unable to Reverse Cardiorespiratory Dysfunction in Experimental Severe Emphysema. Front Cell Dev Biol 9:661385 (2021). PubMed: 34136481
- Djordjevic J et al. Early Onset of Sex-Dependent Mitochondrial Deficits in the Cortex of 3xTg Alzheimer's Mice. Cells 9:N/A (2020). PubMed: 32599904
- Salvi A et al. Involvement of oxidative stress and mitochondrial mechanisms in air pollution-related neurobiological impairments. Neurobiol Stress 12:100205 (2020). PubMed: 32258254
- Snow WM et al. Sex-Specific Effects of Chronic Creatine Supplementation on Hippocampal-Mediated Spatial Cognition in the 3xTg Mouse Model of Alzheimer's Disease. Nutrients 12:N/A (2020). PubMed: 33238473
- Yuan Y et al. Mitochondrial ROS-induced lysosomal dysfunction impairs autophagic flux and contributes to M1 macrophage polarization in a diabetic condition. Clin Sci (Lond) 133:1759-1777 (2019). PubMed: 31383716
- Snow WM et al. Chronic dietary creatine enhances hippocampal-dependent spatial memory, bioenergetics, and levels of plasticity-related proteins associated with NF-?B. Learn Mem 25:54-66 (2018). PubMed: 29339557
- Yang S et al. Mitochondrial dysfunction driven by the LRRK2-mediated pathway is associated with loss of Purkinje cells and motor coordination deficits in diabetic rat model. Cell Death Dis 5:e1217 (2014). WB ; Rat . PubMed: 24810053