The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000.
Use at 2-10 µg/mg of lysate.
功能Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
组织特异性Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
疾病相关Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
序列相似性Belongs to the dynamin family. Contains 1 GED domain.
结构域The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
翻译后修饰Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission. Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity. S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage. Ubiquitination by MARCH5 affects mitochondrial morphology.
细胞定位Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
Dynamin family member proline-rich carboxyl-terminal domain less antibody
Dynamin like protein antibody
Dynamin related protein 1 antibody
Dynamin-1-like protein antibody
Dynamin-like protein 4 antibody
Dynamin-like protein antibody
Dynamin-like protein IV antibody
Dynamin-related protein 1 antibody
DYNIV 11 antibody
Anti-DRP1 antibody 图像
Western blot - Anti-DNM1L antibody (ab123599)
All lanes : Anti-DRP1 antibody (ab123599) at 0.4 µg/ml
Lane 1 : Whole cell lysate
from HeLa cells at 50 µg Lane 2 : Whole cell lysate
from HeLa cells at 15 µg Lane 3 : Whole cell lysate
from 293T cells at 50 µg Lane 4 : Whole cell lysate
from Jurkat cells at 50 µg Lane 5 : Whole cell lysate
from NIH 3T3 cells at 50 µg
Detection of Human DNM1L by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab123599 at 6 µg/mg lysate. Subsequent WB detection was performed using ab123599 at 1 µg/ml.
Anti-DRP1 antibody (ab123599)参考文献
has not yet been referenced specifically in any publications.