Anti-Drosophila FMR1抗体[6A15] (ab10299)
Key features and details
- Mouse monoclonal [6A15] to Drosophila FMR1
- Suitable for: ELISA, ICC/IF, IP, WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
概述
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产品名称
Anti-Drosophila FMR1抗体[6A15] -
描述
小鼠单克隆抗体[6A15] to Drosophila FMR1 -
宿主
Mouse -
经测试应用
适用于: ELISA, ICC/IF, IP, WBmore details -
种属反应性
与反应: Drosophila melanogaster
不与反应: Human -
免疫原
Fusion protein corresponding to Drosophila melanogaster Drosophila FMR1. His-dFMR1 fusion protein (Drosophilia melanogaster) (C-terminal 580aa).
Database link: Q9NFU0 -
常规说明
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Protein A purified from tissue culture supernatant. -
Primary antibody说明
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway. -
克隆
单克隆 -
克隆编号
6A15 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IP | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
Drosophila FMR1 is a RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator of specific mRNAs. Represses translation of futsch to regulate microtubule-dependent synaptic growth and function. Part of the RNA interference (RNAi)-related apparatus; double-stranded RNA induces potent and specific gene silencing. Regulates photoreceptor structure and neurotransmission in the eye. Required for stability of the central pair of microtubules in the spermatid axoneme -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 37528 Drosophila melanogaster
- SwissProt: Q9NFU0 Drosophila melanogaster
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别名
- dFMR antibody
- dFMR1 antibody
- dFXR antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab10299 被引用在 14 文献中.
- Sears JC & Broadie K FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory Circuitry. Cell Rep 33:108266 (2020). PubMed: 33053340
- Lee SK et al. Topoisomerase 3ß interacts with RNAi machinery to promote heterochromatin formation and transcriptional silencing in Drosophila. Nat Commun 9:4946 (2018). PubMed: 30470739
- Bienkowski RS et al. The Conserved, Disease-Associated RNA Binding Protein dNab2 Interacts with the Fragile X Protein Ortholog in Drosophila Neurons. Cell Rep 20:1372-1384 (2017). IP ; Drosophila melanogaster . PubMed: 28793261
- Monyak RE et al. Insulin signaling misregulation underlies circadian and cognitive deficits in a Drosophila fragile X model. Mol Psychiatry 22:1140-1148 (2017). PubMed: 27090306
- Ahmad M et al. RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals. Nucleic Acids Res 44:6335-49 (2016). WB ; Drosophila melanogaster . PubMed: 27257063