概述

  • 产品名称Anti-Drosha抗体
    参阅全部 Drosha 一抗
  • 描述
    山羊多克隆抗体to Drosha
  • 特异性This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide:

    C-KQTDKQKLAQRE

    , corresponding to internal sequence amino acids 811-822 of Human Drosha

  • 阳性对照
    • Human Liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab58589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 159 kDa).

靶标

  • 功能Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.
  • 结构域The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
  • 细胞定位Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DROSHA antibody
    • Drosha double stranded RNA specific endoribonuclease antibody
    • Drosha ribonuclease type III antibody
    • Etohi2 antibody
    • HSA242976 antibody
    • Nuclear RNase III Drosha antibody
    • p241 antibody
    • Protein Drosha antibody
    • Putative protein p241 which interacts with transcription factor Sp1 antibody
    • Putative ribonuclease III antibody
    • RANSE3L antibody
    • Ribonuclease 3 antibody
    • Ribonuclease III antibody
    • Ribonuclease III nuclear antibody
    • Ribonuclease type III nuclear antibody
    • RibonucleaseIII antibody
    • RN 3 antibody
    • RN3 antibody
    • RNase 3 antibody
    • RNase III antibody
    • RNase3 antibody
    • RNASE3L antibody
    • RNaseIII antibody
    • RNASEN antibody
    • RNC_HUMAN antibody
    see all

Anti-Drosha antibody 图像

  • Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 159 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab58589 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58589, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Drosha antibody (ab58589)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for submitting an Abreview of ab58589. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested application, we would like to follow up on this.
Ba...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS)
Total protein in input 2 µg
Specification U2OS
Treatment +/- UVC
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

提交于 May 25 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your response.
ICC stands for for immuno-cytochemistry which requires cells (not tissues); whilst IHC is the abbreviation for immuno-histochemistry.
If you wish to use tissue sections of human gastric samples then you need an an...

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Thank you for your response.
Both antibodies (ab58589 and ab85027) are suitable for IHC-P application and they can detect human Drosha. I would suggest taking a look at the on-line product datasheets to see which one would suit your need best. You...

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Thank you for your enquiry regarding ab12286.
Currently, we have 12 specific publications on this product (ab12286) and the list of these papers can be viewed on the on the on-line datasheet. Since ab12286 has been tested in ICC/IF, IP, WB, ICC, C...

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I am very pleased to hear you would like to accept our offer and test ab12286 in IHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in t...

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I am happy to hear that you would like to take advantage of our 100% Abreview testing discount to test ab12286 in IHC. In order for me to process the discount code I will need a little information. First, I reali...

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That product is guaranteed to work in WB in human or mouse tissue. IHC is not guaranteed as we have not tested it yet. If you are interested in testing this, it may qualify for our 100% abreview discount. This ...

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The product that you have expressed interest in is covered by our Abpromise guarantee. We promise to provide support, replacement or refund of the antibody if you use it according to the information on our datash...

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