概述

  • 产品名称
  • 描述
    兔多克隆抗体to DRIL1
  • 特异性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide (Human) - a portion of DRIL 1 encoded within exon 8.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2500. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
  • 组织特异性
    Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
  • 序列相似性
    Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • 细胞定位
    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARI3A_HUMAN antibody
    • ARID domain containing 3A antibody
    • ARID domain-containing protein 3A antibody
    • ARID3A antibody
    • AT rich interactive domain 3A (BRIGHT- like) protein antibody
    • AT rich interactive domain 3A (BRIGHT-like) antibody
    • AT rich interactive domain-containing protein 3A antibody
    • AT-rich interactive domain-containing protein 3A antibody
    • B cell regulator of IgH transcription antibody
    • B-cell regulator of IgH transcription antibody
    • Bright antibody
    • dead ringer like 1 antibody
    • dead ringer, Drosophila, homolog-like 1 antibody
    • Dead ringer-like protein 1 antibody
    • DRIL1 antibody
    • DRIL3 antibody
    • DRX antibody
    • E2F binding protein 1 antibody
    • E2F-binding protein 1 antibody
    • E2FBP1 antibody
    • Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
    see all

Anti-DRIL1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling DRIL1 with ab4445 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 62 kDa

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

    ECL exposed for 1 second (Western Blot; IP / WB) or 1 minute (Transfected).

     

     

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

Anti-DRIL1 antibody (ab4445)参考文献

This product has been referenced in:
  • Boreström C  et al. E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. J Gen Virol 93:1065-75 (2012). WB, ChIP ; Human . Read more (PubMed: 22302879) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lung cancer cells)
Loading amount
10000 cells
Specification
lung cancer cells
Treatment
RISC-free siRNA and siRNA targeting GATA2
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Dec 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册