The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6, using a pressure boiler
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
May play a role in cell proliferation, differentiation and death.
Highest levels in skeletal muscle, heart and kidney. Low levels in colon, thymus, spleen, small intestine, lung and Leukocytes.
Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.
Polyubiquitinated; which induces proteolytic degradation and is impaired by interaction with RWDD1.