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Chicken Drebrin E protein.
Our Abpromise guarantee covers the use of ab12350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 65 kDa). The difference in the size of the detected band (~110 kDa) and in the predicted (~65 kDa) is due to the large number of negatively charged residues in this protein. Block membrane with 5-10% milk for 1 hour.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration. Fix cells with 4% PFA for 15 minutes. Permeabilise cells for 15 minutes with 0.1% triton X100 in PBS (see Ikeda K et al).|
|ICC/IF||Use at an assay dependent concentration. See Toda et al.|
|IHC-FoFr||1/1000. See Keon et al.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
IHC-P image of Drebrin staining on Human ovarian carcinoma sections using ab12350 (1:100). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab12350 was diluted 1:100 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab12350 for 16 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
This image is courtesy of an Abreview submitted by Dr Martin BroadstockWestern blot showing detection of drebrin in mouse and rat cortical lysates by ab12350.