概述

  • 产品名称DRAQ5™ (5mM)
  • 经测试应用适用于: FM, Flow Cyt, ICC/IFmore details
  • 常规说明DRAQ5™, 1, 5-bis{[2-(di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in fixed or non-fixed/ live cells in combination with common labels such as GFP or FITC. As with any cell­-permeant DNA intercalating probe, DRAQ5™may inhibit cell division in long-term assays and should be tested for any effect. DRAQ5™ can be used in flow cytometry, live cell imaging and cell-based assays and is highly compatible with existing protocols accross a wide range of instrumentation platforms.
    Key features of DRAQ5™ include:
    • Convenient ready-to-use aqueous solution
    • Rapid uptake into living cells, providing a high level of nuclear discrimination
    • No photobleaching effect
    • It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc.
    • No compensation needed with common FITC/GFP + PE combinations in flow cytometry
    • No RNase treatment required

    SPECTRAL PROPERTIES:

    Excitation

    • 647 nm line optimal (Exmax 646 nm)
    • 488, 514, 568 and 633 nm lines, sub-optimal
    • Two-photon excitation (1047 nm) and excitation dark (700-850 nm)

    Emission (instrument dependent):

    • 665 nm to infra-red <800 nm (Emmax 681 nm / 697 nm intercalated with dsDNA)
    • Minimal overlap with vis range e.g. GFP and FITC
    • Em. filters may include 695L, 715LP or 780 LP

    Multi-wavelength imaging with UV / vis fluorochromes

    • No photobleaching effect
    • No fluorescence enhancement upon DNA binding
    • Compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes

性能

    应用

    Our Abpromise guarantee covers the use of ab108410 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.
    Flow Cyt 1/1000 - 1/250. For Gating of Nucleated cells = 5µM; For Cell Cycle Analysis = 20µM
    ICC/IF 1/1000. For Cell-based assays, Immunofluorescence microscopy and In-Cell WB = 5µM
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet section.

    DRAQ5™ (5mM) 图像

    实验方案

    DRAQ5™ (5mM) (ab108410)参考文献

    This product has been referenced in:
    • DeBruhl H  et al. Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis. J Cell Sci 129:430-43 (2016). Read more (PubMed: 26631487) »
    • Welin A  et al. CFP-10 from Mycobacterium tuberculosis selectively activates human neutrophils through a pertussis toxin-sensitive chemotactic receptor. Infect Immun 83:205-13 (2015). Read more (PubMed: 25332123) »

    See all 19 Publications for this product

    Product Wall

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    The information given in page 7 of protocol booklet states "DRAQ5™ staining is accelerated at 37°C and incubation time (5-30 minutes) may be reduced but this should be checked by titration and for ea...

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    Thank you for your email. I am sorry for the inconvenience. Please check the following DRAQ5 specific publications;
    http://www.ncbi.nlm.nih.gov/pubmed/14994375
    http://www.cbm.uam.es/mkfactory.esdomain/webs/cbmso/images/TextBox/DRAQ5.pdf
    ...

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    Vielen Dank für Ihre Anfrage.


    Ich kann bestätigen, dass die DRAQ5 Färbung bei höheren Temperaturen verstärkt wird und deshalb die Inkubationszeit vielleicht angepasst werden muss. Je länger die Inkubationszeit desto mehr Signal erwarten w...

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    1) We would expect the cell staining to diminish after a medium exchange. Despite DRAQ5’s high affinity for dsDNA there is still an equilibrium in place and this will be disturbed by removal of the low residual concentration in the culture medium.
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    DRAQ5 co-staining

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Excellent
    Compatible with FITC, Alexa 568, and Alexa 405 co-staining
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2014

    DRAQ5 Abcam for In Cell Western Assay

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Our lab uses DRAQ5 from Abcam for the In Cell Western Assay. DRAQ5 is used to provide accurate normalization over a broad range of cell densities. Specifically, it can be used for stoichiometric staining of DNA in live or fixed cells. DRAQ5 is used in the 700nm channel to normalize well-to well variation in cell number.
    Picture shows In Cell Wester Assay using DRAQ5 in PC3 and LnCAP prostate cell lines.
    Username

    Abcam user community

    Verified customer

    提交于 Jun 25 2014

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    1. The most important consideration would be keep the cells in the presence of DRAQ5 and not to wash them. The nuclear stain should persist well but the likelihood is that progressively there would be ...

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    ab108410 is not an antibody, it is a DNA dye and can be used on live or fixed cells. The blocking and permeabilization steps are optional. Therefore, you do not need to do them to get a good signal.



    Haben Sie neben der Extinktion auch die Emissionsfilter eingestellt (Emmax 681 nm / 697)?

    Die EX/Em Wellenlänge des Texas Red beträgt 593/618, ist also weit weniger nach rechts verschoben als beim DRAQ5.

    Bitte be...

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    J'ai reçu confirmation que les kits de marquage fluorescent des mitochondries CytoPainter :
    1) peuvent être utilisés sur des cellules vivantes,
    2) ne stoppent pas la division cellulaire,
    3) Ne sont à priori pas to...

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    1-10 of 34 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"