Anti-DRAM抗体[M3-P4B4] (ab64739)

概述

  • 产品名称
    Anti-DRAM抗体[M3-P4B4]
    参阅全部 DRAM 一抗
  • 描述
    小鼠单克隆抗体[M3-P4B4] to DRAM
  • 经测试应用
    适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from within residues 150 - 250 of Human DRAM.

  • 阳性对照
    • This antibody gave a positive signal in HeLa whole cell lysate.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab64739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 26 kDa.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
    • 序列相似性
      Belongs to the DRAM/TMEM150 family.
    • 细胞定位
      Lysosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Damage regulated autophagy modulator antibody
      • Damage-regulated autophagy modulator 1 antibody
      • Damage-regulated autophagy modulator antibody
      • DNA damage-regulated autophagy modulator protein 1 antibody
      • DRAM antibody
      • dram1 antibody
      • DRAM1_HUMAN antibody
      • FLJ11259 antibody
      see all

    Anti-DRAM antibody [M3-P4B4] 图像

    • Anti-DRAM antibody [M3-P4B4] (ab64739) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 20 minutes

    实验方案

    Anti-DRAM antibody [M3-P4B4] (ab64739)参考文献

    ab64739 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for confirming the details.

    I have checked the history of this antibody and can confirm that we haven't received specificity complaints for this product so I am sure protocol troubleshooting will help in getting a clean wes...

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    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and would like to ask few questions in order to understand the problem better;

    ...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (glioma stem cells)
    Specification
    glioma stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x 0.1%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 26 2012

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (HeLa Ms Stomach Ms Kidney Ms Liver)
    Loading amount
    20 µg
    Specification
    HeLa Ms Stomach Ms Kidney Ms Liver
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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