Anti-DRAK2抗体(ab8419)

概述

  • 产品名称Anti-DRAK2抗体
    参阅全部 DRAK2 一抗
  • 描述
    兔多克隆抗体to DRAK2
  • 特异性None to DAP or ZIP kinases. The approximately 70 kDa band visible on Western blots is probably non-related to DRAK2 although it is peptide blockable with ab8454.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    CSKRFRFDDSLPNPHE

    , corresponding to amino acids 351/365 of Human DRAK2. (Peptide available as ab8454.)

  • 阳性对照
    • Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.
  • 常规说明


    Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明DRAK2 Antibody is Ion exchange chromatography purified.
  • Primary antibody说明Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab8419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human DRAK2 peptide (ab8454).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Acts as a positive regulator of apoptosis.
  • 组织特异性Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAP kinase related apoptosis inducing protein antibody
    • DAP kinase related apoptosis inducing protein kinase 2 antibody
    • DAP kinase-related apoptosis-inducing protein kinase 2 antibody
    • Death associated protein kinase related 2 antibody
    • DRAK 2 antibody
    • DRAK2 antibody
    • Serine/threonine kinase 17b antibody
    • Serine/threonine kinase 17b apoptosis inducing antibody
    • Serine/threonine protein kinase 17B antibody
    • Serine/threonine-protein kinase 17B antibody
    • ST17B_HUMAN antibody
    • STK 17B antibody
    • Stk17b antibody
    see all

Anti-DRAK2 antibody 图像

  • Lane 1 : Anti-DRAK2 antibody (ab8419) at 1 µg/ml (DRAK2 antibody)
    Lane 2 : Anti-DRAK2 antibody (ab8419) at 2 µg/ml (DRAK2 antibody)

    Lane 1 : Raji cell lysate
    Lane 2 : Raji cell lysate


    Predicted band size : 43 kDa
  • All lanes : Anti-DRAK2 antibody (ab8419) at 1/500 dilution

    Lane 1 : Jurkat whole cell lysate with absence of blocking peptide
    Lane 2 : Raji whole cell lysate with absence of blocking peptide
    Lane 3 : Jurkat whole cell lysate with presence of blocking peptide
    Lane 4 : Raji whole cell lysate with presence of blocking peptide


    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein.
  • ab8419 at 10µg/ml staining DRAK2 in Jurkat cells by ICC/IF

Anti-DRAK2 antibody (ab8419)参考文献

ab8419 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (B16F10 murine melanoma cell line)
Loading amount 40 µg
Specification B16F10 murine melanoma cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

提交于 Aug 04 2006

Cross-reaction for peptide antibodies is not uncommon, the band at 42 kDa is specific to DRAK2, but the 70 kDa band is likely to be against a different protein from our own internal testing.

Thank you for your enquiry. It is unnecessary to isolate nuclear proteins before running a western blot. You can detect DRAK2 by loading the Jurkat whole cell lysate directly onto the gel.

Thank you for your enquiry. The larger band is probably a cross-reaction to a non-related protein or a different member of the same family. We would suggest trying longer exposure of the membrane to x-ray film (30 minutes to overnight). It is ...

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