概述

  • 产品名称Anti-DRAK1抗体
    参阅全部 DRAK1 一抗
  • 描述
    兔多克隆抗体to DRAK1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    A region between residue 354 and 414 of Human DRAK1 (NP_004751.2)

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

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应用

Our Abpromise guarantee covers the use of ab95184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Acts as a positive regulator of apoptosis. Also acts as a regulator of cellular reactive oxygen species.
  • 组织特异性Highly expressed in placenta. Lower levels in heart, lung, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DAP kinase-related apoptosis-inducing protein kinase 1 antibody
    • death-associated protein kinase-related 1 antibody
    • DRAK1 antibody
    • EC 2.7.11.1 antibody
    • Serine / threonine kinase 17a death associated protein kinase related 1 antibody
    • Serine/threonine kinase 17a antibody
    • Serine/threonine protein kinase 17a antibody
    • Serine/threonine-protein kinase 17A antibody
    • ST17A_HUMAN antibody
    • STK17 A antibody
    • STK17A antibody
    see all

Anti-DRAK1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DRAK1 with ab95184 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DRAK1 antibody (ab95184) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa at 50 µg
    Lane 2 : Whole cell lysate from HeLa at 15 µg
    Lane 3 : Whole cell lysate from HeLa at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa


    Exposure time : 30 seconds
  • Detection of Human DRAK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). Antibodies: ab95184 was used for WB at 0.4 ug/ml and used for IP at 3 ug/mg lysate (lane 2). DRAK1 was also immunoprecipitated by another rabbit DRAK1 antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 10 seconds.

Anti-DRAK1 antibody (ab95184)参考文献

ab95184 has not yet been referenced specifically in any publications.

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