The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Squamous cell carcinoma of the head and neck
Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 µg ab95618 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.