概述

  • 产品名称Anti-DR4抗体
    参阅全部 DR4 一抗
  • 描述
    兔多克隆抗体to DR4
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    ASGTEAAAATPSKVWGSSAG

    , corresponding to amino acids 24/43 of Human DR4 (Peptide available as ab8447.)

  • 阳性对照
    • HeLa or Jurkatwhole cell lysate A 57 kDa band should be detected.

性能

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应用

Our Abpromise guarantee covers the use of ab8415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with Human DR4 peptide (ab8447).

靶标

  • 功能Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • 组织特异性Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • 序列相似性Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo2 antibody
    • CD261 antibody
    • Cytotoxic TRAIL receptor antibody
    • Death receptor 4 antibody
    • DR4 antibody
    • NF related apoptosis-inducing ligand receptor 1 antibody
    • TNF receptor superfamily member 10a antibody
    • TNF-related apoptosis-inducing ligand receptor 1 antibody
    • TNFRSF10A antibody
    • TR10A_HUMAN antibody
    • TRAIL receptor 1 antibody
    • TRAIL-R1 antibody
    • TRAILR 1 antibody
    • TRAILR1 antibody
    • Tumor necrosis factor receptor superfamily member 10A antibody
    • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
    • Tumor necrosis factor receptor superfamily, member 10a antibody
    see all

Anti-DR4 antibody 图像

  • All lanes : Anti-DR4 antibody (ab8415)

    Lane 1 : H: HeLa
    Lane 2 : K: K562
    Lane 3 : J: Jurkat whole cell lysate


    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • ab8415 staining DR4 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes at 16°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in 1.5% Normal horse serum) for 2 hours at 16°C. An undiluted Biotin-conjugated Human pan-specific polyclonal was used as the secondary antibody.

    See Abreview

Anti-DR4 antibody (ab8415)参考文献

This product has been referenced in:
  • Köhler B  et al. Bid participates in genotoxic drug-induced apoptosis of HeLa cells and is essential for death receptor ligands' apoptotic and synergistic effects. PLoS ONE 3:e2844 (2008). WB ; Human . Read more (PubMed: 18665234) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Breast)
Specification Breast
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 16°C
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Abcam user community

Verified customer

提交于 Mar 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cell lines)
Loading amount 20 µg
Specification Breast cell lines
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Nov 19 2010

Thank you for your enquiry. It is highly unlikely that ab8415 cross-reacts with Trail-R2. To make sure, just perform a BLAST search with the immunizing sequence. Trail-R2 will not come up. Unfortunately, we do not have data supporting the specificity o...

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If you are looking for the functional end then it's best to look for the C-terminal as the N-terminal is usually cleaved.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"