概述

  • 产品名称
  • 描述
    兔多克隆抗体to DR4
  • 特异性
    Does not cross react with DR5.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    CDSGKFIYLEDGTGSAVSLE

    , corresponding to amino acids 450/468 of Human DR4 (Peptide available as ab8446.)

  • 阳性对照
    • HeLa whole cell lysate A 57 kDa band should be detected.

性能

应用

Our Abpromise guarantee covers the use of ab8414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with Human DR4 peptide (ab8446).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • 组织特异性
    Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • 序列相似性
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo2 antibody
    • CD261 antibody
    • Cytotoxic TRAIL receptor antibody
    • Death receptor 4 antibody
    • DR4 antibody
    • NF related apoptosis-inducing ligand receptor 1 antibody
    • TNF receptor superfamily member 10a antibody
    • TNF-related apoptosis-inducing ligand receptor 1 antibody
    • TNFRSF10A antibody
    • TR10A_HUMAN antibody
    • TRAIL receptor 1 antibody
    • TRAIL-R1 antibody
    • TRAILR 1 antibody
    • TRAILR1 antibody
    • Tumor necrosis factor receptor superfamily member 10A antibody
    • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
    • Tumor necrosis factor receptor superfamily, member 10a antibody
    see all

Anti-DR4 antibody 图像

  • Anti-DR4 antibody (ab8414) at 1/500 dilution + HeLa total cell lysate

    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of DR4 in human spleen tissue with DR4 antibody at 5 µg/ml.

  • Immunofluorescent analysis of human spleen tissue labeling DR4 with ab8414 at 20 ug/ml.

  • ab8414 at 10µg/ml staining DR4 in Jurkat cells by ICC.

Anti-DR4 antibody (ab8414)参考文献

This product has been referenced in:
  • Park JS  et al. Ethanolic extract of Descurainia sophia seeds sensitizes A549 human lung cancer cells to TRAIL cytotoxicity by upregulating death receptors. BMC Complement Altern Med 16:115 (2016). WB ; Human . Read more (PubMed: 27039295) »
  • Carr RM  et al. Targeting the metabolic pathway of human colon cancer overcomes resistance to TRAIL-induced apoptosis. Cell Death Discov 2:16067 (2016). WB ; Human . Read more (PubMed: 27648301) »

See all 12 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
Yes - TBST
Blocking step
2%BSA/2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Miss. Sandra Peternel

Verified customer

提交于 Jan 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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