A region within synthetic peptide: MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI, corresponding to N terminal amino acids 1-50 of Human DR1
Our Abpromise guarantee covers the use of ab50783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
ChIP | Use at an assay dependent concentration. PubMed: 19114554 | |
ELISA | Use at an assay dependent concentration. Starting ELISA titre using peptide based assay of 1:62500 recommended. |
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