概述

  • 产品名称Anti-DPYD抗体
    参阅全部 DPYD 一抗
  • 描述
    兔多克隆抗体to DPYD
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human DPYD.

    (Peptide available as ab105628.)

  • 阳性对照
    • This antibody gave a positive signal in human liver, kidney and spleen tissue lysates, and Jurkat and THP1 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab95834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 111 kDa).

靶标

  • 功能Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
  • 组织特异性Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
  • 通路Amino-acid biosynthesis; beta-alanine biosynthesis.
  • 疾病相关Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.
  • 序列相似性Belongs to the dihydropyrimidine dehydrogenase family.
    Contains 3 4Fe-4S ferredoxin-type domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DHP antibody
    • DHPDHase antibody
    • Dihydropyrimidine dehydrogenase [NADP(+)] antibody
    • Dihydropyrimidine dehydrogenase [NADP+] antibody
    • Dihydropyrimidine dehydrogenase antibody
    • Dihydrothymine dehydrogenase antibody
    • Dihydrouracil dehydrogenase antibody
    • DPD antibody
    • DPYD antibody
    • DPYD_HUMAN antibody
    • MGC132008 antibody
    • MGC70799 antibody
    • OTTHUMP00000058954 antibody
    see all

Anti-DPYD antibody 图像

  • All lanes : Anti-DPYD antibody (ab95834) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 111 kDa
    Additional bands at : 60 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-DPYD antibody (ab95834)参考文献

ab95834 has not yet been referenced specifically in any publications.

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