概述

  • 产品名称Anti-Dppa4抗体
    参阅全部 Dppa4 一抗
  • 描述
    兔多克隆抗体to Dppa4
  • 特异性The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide derived from within residues 200 to the C-terminus of Mouse Dppa4.

    (Peptide available as ab31647.)

  • 阳性对照
    • Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;

性能

应用

Our Abpromise guarantee covers the use of ab31648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).

靶标

  • 功能May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410091M23Rik antibody
    • Developmental pluripotency associated 4 antibody
    • Developmental pluripotency associated protein 4 antibody
    • Developmental pluripotency-associated protein 4 antibody
    • Dppa 4 antibody
    • DPPA4 antibody
    • DPPA4_HUMAN antibody
    • FLJ10713 antibody
    see all

Anti-Dppa4 antibody 图像

  • All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa (possible IgG,glycosylated form,non-specific binding).

Anti-Dppa4 antibody (ab31648)参考文献

This product has been referenced in:
  • Takahashi K  et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB ; Human . Read more (PubMed: 18035408) »

See 1 Publication for this product

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