The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
Synthetic peptide derived from within residues 200 to the C-terminus of Mouse Dppa4.
(Peptide available as
Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.016% Sodium Azide
Constituents: 0.8% BSA, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
Information by UniProt
Developmental pluripotency associated 4 antibody
Developmental pluripotency associated protein 4 antibody
Western blot - Anti-Dppa4 antibody (ab31648)
All lanes :
Anti-Dppa4 antibody (ab31648) at 1 µg/ml
Lane 1 :
Human Embryonic Stem Cell Lysate
Lane 2 :
Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Rabbit IgG secondary antibody (
) at 1/10000 dilution
Predicted band size:
Observed band size:
36 kDa (
why is the actual band size different from the predicted?
Additional bands at:
48 kDa (possible IgG), 48 kDa (possible glycosylated form), 48 kDa (possible non-specific binding)
ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.
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