Anti-DPP9抗体- Catalytic domain (ab42080)

概述

  • 产品名称Anti-DPP9抗体- Catalytic domain
    参阅全部 DPP9 一抗
  • 描述
    兔多克隆抗体to DPP9 - Catalytic domain
  • 特异性This antibody recognises all three forms of DPP9. This antibody does not recognise other DPP family members.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Baboon
  • 免疫原

    Synthetic peptide corresponding to the catalytic domain of human DPP9.

    (Peptide available as ab44599.)

性能

应用

Our Abpromise guarantee covers the use of ab42080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Recommended dilution 1/1000 with colourimetric substrates, 1/5000 dilution with chemiluminescent substrates. Dilution optimised using Chromogenic detection.
IHC-P 1/50.

靶标

  • 功能Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.
  • 组织特异性Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain.
  • 序列相似性Belongs to the peptidase S9B family. DPPIV subfamily.
  • 细胞定位Cytoplasm > cytosol.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dipeptidyl peptidase 9 antibody
    • Dipeptidyl peptidase IV related protein 2 antibody
    • Dipeptidyl peptidase IV-related protein 2 antibody
    • Dipeptidyl peptidase IX antibody
    • Dipeptidyl peptidase like protein 9 antibody
    • Dipeptidyl peptidase-like protein 9 antibody
    • Dipeptidylpeptidase 9 antibody
    • Dipeptidylpeptidase IX antibody
    • DKFZp762F117 antibody
    • DP 9 antibody
    • DP9 antibody
    • DPLP 9 antibody
    • DPLP9 antibody
    • DPP 9 antibody
    • DPP IX antibody
    • DPP9 antibody
    • DPP9_HUMAN antibody
    • DPRP 2 antibody
    • DPRP-2 antibody
    • DPRP2 antibody
    • FLJ16073 antibody
    see all

Anti-DPP9 antibody - Catalytic domain 图像

  • All lanes : Anti-DPP9 antibody - Catalytic domain (ab42080) at 1/1000 dilution

    Lane 1 : Cell media from human prostate cancer DU145 cells (treated with SF)
    Lane 2 : Cell media from human prostate cancer DU145 cells (treated with RA)
    Lane 3 : Cell media from human prostate cancer DU145 cells (treated with IL1-beta)


    Predicted band size : 98 kDa
  • Ab42080 staining Human normal placenta. Staining is localised to the membrane and cytoplasm.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab42080 staining DPP9 in bovine testicular tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA buffer pH 9.0 and then the sample was blocked with 3% hydrogen peroxide in TBS for 10 minutes at 25°C. The sections were treated for 10 min with 3% H2O2 in TBS to block endogenous peroxidase activity and incubated subsequently for 30 min with 10% nonimmune goat serum to minimize nonspecific antibody binding. The primary antibody (1/50) was incubated with sample in TBS, 0.3% Triton X-100, 0.1 % BSA for 16 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.

    See Abreview

  • ab42080 at 1/50 dilution staining DPP9 in rat brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, permeabilized in 0.2% saponin prior to blocking in 3% H2O2 in PBS for 10 minutes at RT. The sample was incubated with ab42080 for 1 hour. A HRP conjugated goat polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab42080 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DPP9 antibody - Catalytic domain (ab42080)参考文献

This product has been referenced in:
  • Justa-Schuch D  et al. DPP9 is a novel component of the N-end rule pathway targeting the tyrosine kinase Syk. Elife 5:N/A (2016). WB ; Human . Read more (PubMed: 27614019) »
  • Justa-Schuch D  et al. The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus. Cell Mol Life Sci 71:3611-26 (2014). WB . Read more (PubMed: 24562348) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Baboon Tissue sections (Colon, muscle, cerebellum, skin, testes)
Specification Colon, muscle, cerebellum, skin, testes
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Universal Decloaker
Permeabilization Yes - Saponin
Blocking step H2O2 in PBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Miss. Denise Yu

Verified customer

提交于 Feb 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon, liver, gallbladder)
Specification Colon, liver, gallbladder
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Universal Decloaker
Permeabilization Yes - 0.2% saponin
Blocking step H2O2 in PBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Miss. Denise Yu

Verified customer

提交于 Nov 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 5 µg
Specification HeLa cells
Treatment siRNA
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Purified protein (testes)
Loading amount 0.32 µg
Specification testes
Gel Running Conditions Reduced Denaturing (7,5% gel)
Blocking step 0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

提交于 Jun 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (testis)
Specification testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
Permeabilization No
Blocking step 3% H2O2 in TBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

提交于 Jun 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"