Anti-DPP6抗体- Catalytic domain (ab41811)

概述

  • 产品名称Anti-DPP6抗体- Catalytic domain
    参阅全部 DPP6 一抗
  • 描述
    兔多克隆抗体to DPP6 - Catalytic domain
  • 特异性This antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Synthetic peptide based on the catalytic domain of human DPP6.

    (Peptide available as ab44770.)

性能

应用

Our Abpromise guarantee covers the use of ab41811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
WB
  • 应用说明WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
    • 细胞定位Cell Membrane; single-pass type II membrane protein
    • 数据库链接
    • 别名
      • Dipeptidyl aminopeptidase IV related protein antibody
      • Dipeptidyl aminopeptidase like protein 6 antibody
      • Dipeptidyl aminopeptidase related protein antibody
      • Dipeptidyl peptidase 6 antibody
      • Dipeptidyl peptidase IV like protein antibody
      • Dipeptidyl peptidase IV related protein antibody
      • Dipeptidylpeptidase 6 antibody
      • Dipeptidylpeptidase VI antibody
      • DPP VI antibody
      • DPPX antibody
      • VF2 antibody
      see all

    Anti-DPP6 antibody - Catalytic domain 图像

    • All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution

      Lane 1 : Mouse Brain
      Lane 2 : DU145 / IL1-beta Cell media


      Predicted band size : 98 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa (possible truncated form).
    • ab41811 staining DPP6 - Catalytic domain in zebrafish brain tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in paraformaldehyde, blocked with 2% serum for 2 hours at 24°C and then incubated with ab41811 at a 1/25 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

      See Abreview

    Anti-DPP6 antibody - Catalytic domain (ab41811)参考文献

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Zebrafish Tissue lysate - whole (Neuronal tissue)
    Gel Running Conditions Reduced Denaturing (7.5%)
    Treatment RIPA buffer with protease inhibitor
    Specification Neuronal tissue
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 May 24 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Zebrafish Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Username

    Dr. Brent Bill

    Verified customer

    提交于 Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"