概述

  • 产品名称Anti-DP2抗体
    参阅全部 DP2 一抗
  • 描述
    兔多克隆抗体to DP2
  • 特异性ab26260 recognises TFDP2.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: MIISTPQRLT SSGSVLIGSP YTPAPAMVTQ THIAEATGWV PGDRKRARKF, corresponding to a region within N terminal amino acids 1-50 of Human DP2

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab26260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.

靶标

  • 功能Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
  • 组织特异性High levels in heart and skeletal muscle. Also found in placenta, kidney, brain, lung and liver. The presence as well as the abundance of the different transcripts appear to vary significantly in different tissues and cell lines.
  • 序列相似性Belongs to the E2F/DP family.
  • 翻译后修饰Ser-24 is probably phosphorylated by CDK2.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DP2 antibody
    • E2F dimerization partner 2 antibody
    • Tfdp2 antibody
    • TFDP2_HUMAN antibody
    • Transcription factor Dp 2 antibody
    • Transcription factor Dp-2 antibody
    see all

Anti-DP2 antibody 图像

  • Anti-DP2 antibody (ab26260) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Staining of human kidney tissue with ab26260 (4-8 ug/ml). Positive labelled epithelial cells of renal tubule are indicated with arrows. 400X magnification.

Anti-DP2 antibody (ab26260)参考文献

ab26260 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"